Typical Testing Data/Standard Curve (for reference only)
Rat TIMP-2 ELISA Kit | TIMP-2 elisa kit
Rat TIMP-2(Tissue Inhibitors of Metalloproteinase 2)ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat TIMP-2. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat TIMP-2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat TIMP-2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Rat TIMP-2. You can calculate the concentration of Rat TIMP-2 in the samples by comparing the OD of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI Description
acts as an inhibitor of metallopeptidase activity; may play a role in tissue remodeling during spermatogenesis [RGD, Feb 2006]
Uniprot Description
TIMP2: Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. Known to act on MMP-1, MMP-2, MMP-3, MMP-7, MMP-8, MMP-9, MMP-10, MMP-13, MMP-14, MMP-15, MMP-16 and MMP-19. Interacts (via the C-terminal) with MMP2 (via the C- terminal PEX domain); the interaction inhibits the MMP2 activity. Down-regulated by TGFB1. Belongs to the protease inhibitor I35 (TIMP) family.
Protein type: Secreted, signal peptide; Motility/polarity/chemotaxis; Secreted; Extracellular matrix; Inhibitor; Cell development/differentiation
Cellular Component: extracellular space; proteinaceous extracellular matrix; neuron projection; growth cone; cell surface; cell soma; basement membrane; nucleus
Molecular Function: integrin binding; metalloendopeptidase inhibitor activity; protease binding; metal ion binding; enzyme activator activity
Biological Process: response to drug; negative regulation of proteolysis; positive regulation of catalytic activity; regulation of Rap protein signal transduction; negative regulation of metalloenzyme activity; central nervous system development; regulation of neuron differentiation; regulation of cAMP metabolic process; response to hormone stimulus; negative regulation of membrane protein ectodomain proteolysis; negative regulation of cell proliferation; positive regulation of MAPKKK cascade; response to cytokine stimulus; regulation of MAPKKK cascade; negative regulation of Ras protein signal transduction; spermatogenesis; negative regulation of mitotic cell cycle; positive regulation of adenylate cyclase activity; positive regulation of neuron differentiation; aging
Research Articles on TIMP-2
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Product Notes
The Rat TIMP-2 timp2 (Catalog #AAA2504314) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2504314 ELISA Kit recognizes Rat TIMP-2. It is sometimes possible for the material contained within the vial of "TIMP-2, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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