Typical Testing Data/Standard Curve (for reference only)
Porcine Tissue inhibitors of metalloproteinase 2 ELISA Kit | TIMP-2 elisa kit
Porcine Tissue inhibitors of metalloproteinase 2 ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
PRINCIPLE OF THE ASSAY TIMP-2 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for TIMP-2. Standards or samples are then added to the microtiter plate wells and TIMP-2 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of TIMP-2 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for TIMP-2 are added to each well to “sandwich” the TIMP-2 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain TIMP-2 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The TIMP-2 concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
acts as an inhibitor of metallopeptidase activity; may play a role in tissue remodeling during spermatogenesis [RGD, Feb 2006]
Uniprot Description
TIMP2: Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. Known to act on MMP-1, MMP-2, MMP-3, MMP-7, MMP-8, MMP-9, MMP-10, MMP-13, MMP-14, MMP-15, MMP-16 and MMP-19. Interacts (via the C-terminal) with MMP2 (via the C- terminal PEX domain); the interaction inhibits the MMP2 activity. Down-regulated by TGFB1. Belongs to the protease inhibitor I35 (TIMP) family.
Protein type: Secreted; Inhibitor; Extracellular matrix; Secreted, signal peptide; Cell development/differentiation; Motility/polarity/chemotaxis
Cellular Component: proteinaceous extracellular matrix; extracellular space; neuron projection; cell surface; growth cone; cell soma; basement membrane; nucleus
Molecular Function: integrin binding; metalloendopeptidase inhibitor activity; protease binding; metal ion binding; enzyme activator activity
Biological Process: positive regulation of catalytic activity; response to drug; regulation of Rap protein signal transduction; negative regulation of proteolysis; negative regulation of metalloenzyme activity; central nervous system development; regulation of cAMP metabolic process; regulation of neuron differentiation; response to hormone stimulus; negative regulation of membrane protein ectodomain proteolysis; negative regulation of cell proliferation; positive regulation of MAPKKK cascade; regulation of MAPKKK cascade; response to cytokine stimulus; negative regulation of Ras protein signal transduction; spermatogenesis; negative regulation of mitotic cell cycle; positive regulation of adenylate cyclase activity; positive regulation of neuron differentiation; aging
Research Articles on TIMP-2
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Product Notes
The Porcine TIMP-2 timp2 (Catalog #AAA737317) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA737317 ELISA Kit recognizes Porcine TIMP-2. It is sometimes possible for the material contained within the vial of "Tissue inhibitors of metalloproteinase 2, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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