Typical Testing Data/Standard Curve (for reference only)
Bovine Tissue inhibitors of metalloproteinase 2 ELISA Kit | TIMP-2 elisa kit
Bovine Tissue inhibitors of metalloproteinase 2 ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
INTENDED USE This TIMP2 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Bovine TIMP2. This ELISA kit for research use only!
PRINCIPLE OF THE ASSAY TIMP2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-TIMP2 antibody and an TIMP2-HRP conjugate. The assay sample and buffer are incubated together with TIMP2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the TIMP2 concentration since TIMP2 from samples and TIMP2-HRP conjugate compete for the anti-TIMP2 antibody binding site. Since the number of sites is limited, as more sites are occupied by TIMP2 from the sample, fewer sites are left to bind TIMP2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The TIMP2 concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
acts as an inhibitor of metallopeptidase activity; may play a role in tissue remodeling during spermatogenesis [RGD, Feb 2006]
Uniprot Description
TIMP2: Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. Known to act on MMP-1, MMP-2, MMP-3, MMP-7, MMP-8, MMP-9, MMP-10, MMP-13, MMP-14, MMP-15, MMP-16 and MMP-19. Interacts (via the C-terminal) with MMP2 (via the C- terminal PEX domain); the interaction inhibits the MMP2 activity. Down-regulated by TGFB1. Belongs to the protease inhibitor I35 (TIMP) family.
Protein type: Secreted; Motility/polarity/chemotaxis; Extracellular matrix; Inhibitor; Cell development/differentiation; Secreted, signal peptide
Cellular Component: proteinaceous extracellular matrix; extracellular space; neuron projection; growth cone; cell surface; cell soma; basement membrane; nucleus
Molecular Function: metalloendopeptidase inhibitor activity; integrin binding; protease binding; metal ion binding; enzyme activator activity
Biological Process: response to drug; negative regulation of proteolysis; positive regulation of catalytic activity; regulation of Rap protein signal transduction; negative regulation of metalloenzyme activity; central nervous system development; regulation of neuron differentiation; regulation of cAMP metabolic process; response to hormone stimulus; negative regulation of membrane protein ectodomain proteolysis; negative regulation of cell proliferation; positive regulation of MAPKKK cascade; regulation of MAPKKK cascade; response to cytokine stimulus; negative regulation of Ras protein signal transduction; spermatogenesis; negative regulation of mitotic cell cycle; positive regulation of adenylate cyclase activity; positive regulation of neuron differentiation; aging
Research Articles on TIMP-2
Similar Products
Product Notes
The Bovine TIMP-2 timp2 (Catalog #AAA736572) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA736572 ELISA Kit recognizes Bovine TIMP-2. It is sometimes possible for the material contained within the vial of "Tissue inhibitors of metalloproteinase 2, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
