Canine Tissue inhibitors of metalloproteinase 2 ELISA Kit | TIMP-2 elisa kit

Canine Tissue inhibitors of metalloproteinase 2 ELISA Kit

Cat. Num. AAA742787

• Reactivity: Canine
• Synonyms: Tissue inhibitors of metalloproteinase 2; Canine Tissue inhibitors of metalloproteinase 2 ELISA Kit; TIMP-2 elisa kit

• Reactivity: Canine

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Sandwich
• Detection Range: 25-500ng/mL
• Sensitivity: 1.0ng/mL
• Intended Uses: This TIMP-2 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Canine TIMP-2. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for TIMP-2 elisa kit

Principle of the assay: TIMP-2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-TIMP-2 antibody and an TIMP-2-HRP conjugate. The assay sample and buffer are incubated together with TIMP-2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the TIMP-2 concentration since TIMP-2 from samples and TIMP-2-HRP conjugate compete for the anti-TIMP-2 antibody binding site. Since the number of sites is limited, as more sites are occupied by TIMP-2 from the sample, fewer sites are left to bind TIMP-2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The TIMP-2 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for TIMP-2 elisa kit

Cancer

NCBI and Uniprot Product Information

• NCBI GI #: 619233
• NCBI GeneID: 29543
• Molecular Weight: 24,356 Da
• NCBI Official Full Name: tissue inhibitor of metalloproteinase type 2
• NCBI Official Synonym Full Names: TIMP metallopeptidase inhibitor 2
• NCBI Official Symbol: Timp2
• NCBI Protein Information: metalloproteinase inhibitor 2; TIMP-2; tissue inhibitor of metalloproteinase 2; tissue inhibitor of metalloproteinases 2
• UniProt Protein Name: Metalloproteinase inhibitor 2
• UniProt Gene Name: Timp2
• UniProt Synonym Gene Names: Timp-2; TIMP-2
• UniProt Entry Name: TIMP2_RAT

NCBI Description

acts as an inhibitor of metallopeptidase activity; may play a role in tissue remodeling during spermatogenesis [RGD, Feb 2006]

Uniprot Description

TIMP2: Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. Known to act on MMP-1, MMP-2, MMP-3, MMP-7, MMP-8, MMP-9, MMP-10, MMP-13, MMP-14, MMP-15, MMP-16 and MMP-19. Interacts (via the C-terminal) with MMP2 (via the C- terminal PEX domain); the interaction inhibits the MMP2 activity. Down-regulated by TGFB1. Belongs to the protease inhibitor I35 (TIMP) family.

Protein type: Cell development/differentiation; Inhibitor; Secreted; Secreted, signal peptide; Extracellular matrix; Motility/polarity/chemotaxis

Cellular Component: extracellular space; proteinaceous extracellular matrix; neuron projection; growth cone; cell surface; cell soma; basement membrane; nucleus

Molecular Function: metalloendopeptidase inhibitor activity; integrin binding; protease binding; metal ion binding; enzyme activator activity

Biological Process: regulation of Rap protein signal transduction; positive regulation of catalytic activity; negative regulation of proteolysis; response to drug; negative regulation of metalloenzyme activity; central nervous system development; regulation of neuron differentiation; regulation of cAMP metabolic process; response to hormone stimulus; negative regulation of membrane protein ectodomain proteolysis; negative regulation of cell proliferation; positive regulation of MAPKKK cascade; response to cytokine stimulus; regulation of MAPKKK cascade; negative regulation of Ras protein signal transduction; negative regulation of mitotic cell cycle; spermatogenesis; positive regulation of adenylate cyclase activity; positive regulation of neuron differentiation; aging