Typical Testing Data/Standard Curve (for reference only)
Rat TGFBI ELISA Kit | TGFBI elisa kit
Rat TGFBI ELISA Kit
Gene Names
Tgfbi; BIGH3; Big-h3
Reactivity
Rat
Synonyms
TGFBI; Rat TGFBI ELISA Kit; BIGH3; Transforming growth factor-beta-induced protein ig-h3; Beta ig-h3; Kerato-epithelin; RGD-containing collagen-associated protein; RGD-CAP; TGFBI elisa kit
Reactivity
Rat
Specificity
The Rat TGFBI ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Rat TGFBI proteins within the range of 156 pg/ml - 10000 pg/ml.
Sequence Length
685
Samples
Cell culture supernates, serum, plasma
Sensitivity
< 15 pg/ml
Preparation and Storage
Shipped and store at 4 degree C for 6 months, store at -20 degree C for one year. Avoid freeze/thaw cycles.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for TGFBI elisa kit
Principle of the Assay: The MyBioSource' Rat TGFBI ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Rat TGFBI in Cell Culture Supernates, Serum, Plasma. This assay employs an antibody specific for Rat TGFBI coated on a 96-well plate. Standards and samples are pipetted into the wells and TGFBI present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Rat TGFBI antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of TGFBI bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Background/Introduction: Transforming growth factor, beta-induced, 68kDa, also known as TGFBI (initially called BIGH3, BIG-H3), is a protein which in humans is encoded by the TGFBI gene. It is mapped to 5q31.1. This gene encodes an RGD-containing protein that binds to type I, II and IV collagens. The RGD motif is found in many extracellular matrix proteins modulating cell adhesion and serves as a ligand recognition sequence for several integrins. This protein plays a role in cell-collagen interactions and may be involved in endochondrial bone formation in cartilage. It is induced by transforming growth factor-beta and acts to inhibit cell adhesion.
Background/Introduction: Transforming growth factor, beta-induced, 68kDa, also known as TGFBI (initially called BIGH3, BIG-H3), is a protein which in humans is encoded by the TGFBI gene. It is mapped to 5q31.1. This gene encodes an RGD-containing protein that binds to type I, II and IV collagens. The RGD motif is found in many extracellular matrix proteins modulating cell adhesion and serves as a ligand recognition sequence for several integrins. This protein plays a role in cell-collagen interactions and may be involved in endochondrial bone formation in cartilage. It is induced by transforming growth factor-beta and acts to inhibit cell adhesion.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
74,753 Da
NCBI Official Full Name
transforming growth factor-beta-induced protein ig-h3
NCBI Official Synonym Full Names
transforming growth factor, beta induced
NCBI Official Symbol
Tgfbi
NCBI Official Synonym Symbols
BIGH3; Big-h3
NCBI Protein Information
transforming growth factor-beta-induced protein ig-h3
UniProt Protein Name
Protein Tgfbi
Protein Family
UniProt Gene Name
Tgfbi
UniProt Entry Name
D4A8G5_RAT
NCBI Description
mouse homolog is an extracellular matrix protein; human homolog is associated with hereditary corneal dystrophies [RGD, Feb 2006]
Research Articles on TGFBI
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Product Notes
The Rat TGFBI tgfbi (Catalog #AAA8248487) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA8248487 ELISA Kit recognizes Rat TGFBI. It is sometimes possible for the material contained within the vial of "TGFBI, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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