Typical Testing Data/Standard Curve (for reference only)
(Rat beta IG-H3/TGFBI PicoKine ELISA Kit standard curve )
Rat beta IG-H3/TGFBI ELISA Kit | TGFBI elisa kit
Rat beta IG-H3/TGFBI PicoKine ELISA Kit
O.D.: |0.010|0.031|0.064|0.137|0.376|0.922|1.879|2.599
n: |16|16|16|24|24|24
Mean(pg/ml): |451|1349|5410|563|1588|5523
Standard Deviation: |18.49|70.15|373.29|24.21|87.34|397.66
CV(%): |4.1|5.2|6.9|4.3|5.5|7.2
Typical Testing Data/Standard Curve (for reference only)
(Rat beta IG-H3/TGFBI PicoKine ELISA Kit standard curve )
Typical Testing Data/Standard Curve (for reference only)
(Rat beta IG-H3/TGFBI PicoKine ELISA Kit standard curve )
Background: Transforming growth factor, beta-induced, 68kDa, also known as TGFBI (initially called BIGH3, BIG-H3), is a protein which in humans is encoded by the TGFBI gene. It is mapped to 5q31.1. This gene encodes an RGD-containing protein that binds to type I, II and IV collagens. The RGD motif is found in many extracellular matrix proteins modulating cell adhesion and serves as a ligand recognition sequence for several integrins. This protein plays a role in cell-collagen interactions and may be involved in endochondrial bone formation in cartilage. It is induced by transforming growth factor-beta and acts to inhibit cell adhesion.
Principle: Rat beta IG-H3 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from Mouse specific for beta IG-H3 has been precoated onto 96-well plates. Standards (Expression system for standard: NSO, Immunogen sequence: G24-G685) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for beta IG-H3 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substRate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Rat beta IG-H3 amount of sample captured in plate.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes an RGD-containing protein that binds to type I, II and IV collagens. The RGD motif is found in many extracellular matrix proteins modulating cell adhesion and serves as a ligand recognition sequence for several integrins. This protein plays a role in cell-collagen interactions and may be involved in endochondrial bone formation in cartilage. The protein is induced by transforming growth factor-beta and acts to inhibit cell adhesion. Mutations in this gene are associated with multiple types of corneal dystrophy. [provided by RefSeq, Jul 2008]
Uniprot Description
betaIG-H3: Binds to type I, II, and IV collagens. This adhesion protein may play an important role in cell-collagen interactions. In cartilage, may be involved in endochondral bone formation. By TGFB1. Highly expressed in the corneal epithelium.
Protein type: Cell adhesion; Extracellular matrix; Secreted; Secreted, signal peptide
Chromosomal Location of Human Ortholog: 5q31.1
Cellular Component: extracellular matrix; extracellular region; extracellular space; plasma membrane; proteinaceous extracellular matrix; trans-Golgi network
Molecular Function: collagen binding; protein binding
Biological Process: angiogenesis; cellular protein metabolic process; extracellular matrix organization and biogenesis; negative regulation of cell adhesion
Disease: Corneal Dystrophy Of Bowman Layer, Type I; Corneal Dystrophy Of Bowman Layer, Type Ii; Corneal Dystrophy, Avellino Type; Corneal Dystrophy, Epithelial Basement Membrane; Corneal Dystrophy, Groenouw Type I; Corneal Dystrophy, Lattice Type I; Corneal Dystrophy, Lattice Type Iiia
Research Articles on TGFBI
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Product Notes
The Rat TGFBI tgfbi (Catalog #AAA178757) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA178757 ELISA Kit recognizes Rat TGFBI. It is sometimes possible for the material contained within the vial of "beta IG-H3/TGFBI, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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