Western Blot (WB)
(Western Blot analysis of extracellularmedia LEC grown for 24 h under normoxia (N), hypoxia (H) or hypoxia and reoxygenation (H/N) for 6 h with TGFBI / BIGH3 Monoclonal Antibody . Silver staining of the membranes was used as loading control.)
Mouse anti-Human, Mouse TGFBI Monoclonal Antibody | anti-TGFBI antibody
TGFBI Monoclonal Antibody
Gene Names
TGFBI; CSD; CDB1; CDG2; CSD1; CSD2; CSD3; EBMD; LCD1; BIGH3; CDGG1
Reactivity
Human, Mouse
Applications
Western Blot, Immunohistochemistry, ELISA
Purity
Caprylic acid/ammonium sulfate precipitation
Synonyms
TGFBI; Monoclonal Antibody; TGFBI Monoclonal Antibody; >RGD containing collagen associated protein; AI181842; AI747162; Beta ig; Beta ig h3; Beta ig-h3; BGH3; Big h3; BIGH3; CDB1; CDG2; CDGG1; CSD; CSD1; CSD2; CSD3; EBMD; Kerato epithelin; Kerato-epithelin; LCD1; MGC150270; RGD CAP; RGD-CAP; RGD-containing collagen-associated protein; TGFBI transforming growth factor; beta induced; 68kDa; Transforming growth factor beta induced protein ig h3; Transforming growth factor-beta-induced protein ig-h3; anti-TGFBI antibody
Host
Mouse
Reactivity
Human, Mouse
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
654
Purity/Purification
Caprylic acid/ammonium sulfate precipitation
Concentration
1mg/mL (varies by lot)
Sequence Length
683
Applicable Applications for anti-TGFBI antibody
Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)
Application Notes
WB: 1:200-1:2000
IHC: 1:20-1:200
IHC: 1:20-1:200
Immunogen
Fusion protein of TGFBI / BIGH3
Buffer
PBS with 0.1% sodium azide, 50% glycerol, PH7.3
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Avoid freeze / thaw cycles.
Western Blot (WB)
(Western Blot analysis of extracellularmedia LEC grown for 24 h under normoxia (N), hypoxia (H) or hypoxia and reoxygenation (H/N) for 6 h with TGFBI / BIGH3 Monoclonal Antibody . Silver staining of the membranes was used as loading control.)
Western Blot (WB)
(Western Blot analysis of extracellularmedia LEC grown for 24 h under normoxia (N), hypoxia (H) or hypoxia and reoxygenation (H/N) for 6 h with TGFBI / BIGH3 Monoclonal Antibody . Silver staining of the membranes was used as loading control.)
Related Product Information for anti-TGFBI antibody
TGFBI, also named as BIGH3, Kerato-epithelin and RGD-CAP, binds to type I, II, and IV collagens. TGFBI is an adhesion protein which may play an important role in cell-collagen interactions. In cartilage, it may be involved in endochondral bone formation. TGFBI is an extracellular matrix adaptor protein, it has been reported to be differentially expressed in transformed tissues. TGFBI is a predictive factor of the response to chemotherapy, and suggest the use of TGFBI-derived peptides as possible therapeutic adjuvants for the enhancement of responses to chemotherapy. Defects in TGFBI are the cause of epithelial basement membrane corneal dystrophy (EBMD). Defects in TGFBI are the cause of corneal dystrophy Groenouw type 1 (CDGG1). Defects in TGFBI are the cause of corneal dystrophy lattice type 1 (CDL1). Defects in TGFBI are a cause of corneal dystrophy Thiel-Behnke type (CDTB). Defects in TGFBI are the cause of Reis-Buecklers corneal dystrophy (CDRB). Defects in TGFBI are the cause of lattice corneal dystrophy type 3A (CDL3A). Defects in TGFBI are the cause of Avellino corneal dystrophy (ACD).
Product Categories/Family for anti-TGFBI antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Calculated: 75kDa
Observed: 68kDa
Observed: 68kDa
NCBI Official Full Name
transforming growth factor-beta-induced protein ig-h3
NCBI Official Synonym Full Names
transforming growth factor beta induced
NCBI Official Symbol
TGFBI
NCBI Official Synonym Symbols
CSD; CDB1; CDG2; CSD1; CSD2; CSD3; EBMD; LCD1; BIGH3; CDGG1
NCBI Protein Information
transforming growth factor-beta-induced protein ig-h3
UniProt Protein Name
Transforming growth factor-beta-induced protein ig-h3
Protein Family
UniProt Gene Name
TGFBI
UniProt Synonym Gene Names
BIGH3; Beta ig-h3; RGD-CAP
NCBI Description
This gene encodes an RGD-containing protein that binds to type I, II and IV collagens. The RGD motif is found in many extracellular matrix proteins modulating cell adhesion and serves as a ligand recognition sequence for several integrins. This protein plays a role in cell-collagen interactions and may be involved in endochondrial bone formation in cartilage. The protein is induced by transforming growth factor-beta and acts to inhibit cell adhesion. Mutations in this gene are associated with multiple types of corneal dystrophy. [provided by RefSeq, Jul 2008]
Uniprot Description
Plays a role in cell adhesion (PubMed:8024701). May play a role in cell-collagen interactions ().
Research Articles on TGFBI
Similar Products
Product Notes
The TGFBI tgfbi (Catalog #AAA2540240) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The TGFBI Monoclonal Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's TGFBI can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA). WB: 1:200-1:2000 IHC: 1:20-1:200. Researchers should empirically determine the suitability of the TGFBI tgfbi for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TGFBI, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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