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Typical Testing Data/Standard Curve (for reference only)

Rat TGFBI ELISA Kit | TGFBI elisa kit

Rat TGFBI (Transforming Growth Factor Beta Induced Protein) ELISA Kit

Gene Names
TGFBI; CSD; CDB1; CDG2; CSD1; CSD2; CSD3; EBMD; LCD1; BIGH3; CDGG1
Reactivity
Rat
Synonyms
TGFBI; Rat TGFBI (Transforming Growth Factor Beta Induced Protein) ELISA Kit; TGFBI elisa kit
Ordering
For Research Use Only!
Reactivity
Rat
Specificity
This kit recognizes natural and recombinant Rat TGF betaI. No significant cross-reactivity or interference between Rat TGF betaI and analogues was observed.
Sequence Length
683
Samples
Serum, Plasma, Biological Fluids
Assay Type
Sandwich
Detection Range
31.25-2000pg/mL
Sensitivity
Min: 18.75pg/mL; Max: 2000pg/mL
Intended Uses
This ELISA kit applies to the in vitro quantitative determination of Rat TGF betaI concentrations in serum, plasma and other biological fluids.
Preparation and Storage
Store at 4 degree C.

Typical Testing Data/Standard Curve (for reference only)

Typical Testing Data/Standard Curve (for reference only)
Related Product Information for TGFBI elisa kit
Principle of the assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to TGF betaI. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for TGF betaI and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain TGF betaI, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of TGF betaI. You can calculate the concentration of TGF betaI in the samples by comparing the OD of the samples to the standard curve.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
74,681 Da
NCBI Official Full Name
transforming growth factor-beta-induced protein ig-h3
NCBI Official Synonym Full Names
transforming growth factor, beta-induced, 68kDa
NCBI Official Symbol
TGFBI
NCBI Official Synonym Symbols
CSD; CDB1; CDG2; CSD1; CSD2; CSD3; EBMD; LCD1; BIGH3; CDGG1
NCBI Protein Information
transforming growth factor-beta-induced protein ig-h3; RGD-CAP; RGD-containing collagen-associated protein; beta ig-h3; kerato-epithelin; transforming growth factor, beta-induced, 68kD
UniProt Protein Name
Transforming growth factor-beta-induced protein ig-h3
UniProt Gene Name
TGFBI
UniProt Synonym Gene Names
BIGH3; Beta ig-h3; RGD-CAP
UniProt Entry Name
BGH3_HUMAN

NCBI Description

This gene encodes an RGD-containing protein that binds to type I, II and IV collagens. The RGD motif is found in many extracellular matrix proteins modulating cell adhesion and serves as a ligand recognition sequence for several integrins. This protein plays a role in cell-collagen interactions and may be involved in endochondrial bone formation in cartilage. The protein is induced by transforming growth factor-beta and acts to inhibit cell adhesion. Mutations in this gene are associated with multiple types of corneal dystrophy. [provided by RefSeq, Jul 2008]

Uniprot Description

betaIG-H3: Binds to type I, II, and IV collagens. This adhesion protein may play an important role in cell-collagen interactions. In cartilage, may be involved in endochondral bone formation. By TGFB1. Highly expressed in the corneal epithelium.

Protein type: Cell adhesion; Secreted, signal peptide; Extracellular matrix; Secreted

Chromosomal Location of Human Ortholog: 5q31

Cellular Component: extracellular matrix; proteinaceous extracellular matrix; extracellular space; extracellular region; plasma membrane; basement membrane; trans-Golgi network

Molecular Function: collagen binding; integrin binding; protein binding; extracellular matrix binding

Biological Process: cell proliferation; extracellular matrix organization and biogenesis; visual perception; response to stimulus; chondrocyte differentiation; negative regulation of cell adhesion; angiogenesis; cell adhesion

Disease: Corneal Dystrophy, Epithelial Basement Membrane; Corneal Dystrophy, Lattice Type Iiia; Corneal Dystrophy Of Bowman Layer, Type Ii; Corneal Dystrophy, Avellino Type; Corneal Dystrophy, Lattice Type I; Corneal Dystrophy Of Bowman Layer, Type I; Corneal Dystrophy, Groenouw Type I

Research Articles on TGFBI

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Product Notes

The Rat TGFBI tgfbi (Catalog #AAA2510467) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2510467 ELISA Kit recognizes Rat TGFBI. It is sometimes possible for the material contained within the vial of "TGFBI, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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