Mouse Phosphoinositide 3 Kinase Monoclonal Antibody | anti-PIK3R2 antibody

Phosphoinositide 3 Kinase, p85 beta (PI-3 Kinase p85 beta, PI3 Kinase p85 beta Subunit, PI3 Kinase p85 Subunit beta, PI3K, p85 beta, P85B, Phosphatidylinositol 3 Kinase Regulatory beta Subunit, Phosphatidylinositol 3 Kinase Regulatory Subunit beta, Phosph

Cat. Num. AAA6251173

• Reactivity: Bovine, Human, Monkey, Mouse, Rat
• Applications: Immunohistochemistry, Immunoprecipitation, Western Blot
• Purity: Purified by Protein G Affinity Chromatography
• Synonyms: Phosphoinositide 3 Kinase; Monoclonal Antibody; p85 beta (PI-3 Kinase p85 beta; PI3 Kinase p85 beta Subunit; PI3 Kinase p85 Subunit beta; PI3K; p85 beta; P85B; Phosphatidylinositol 3 Kinase Regulatory beta Subunit; Phosphatidylinositol 3 Kinase Regulatory Subunit beta; Phosph; anti-PIK3R2 antibody

• Host: Mouse
• Reactivity: Bovine, Human, Monkey, Mouse, Rat
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 10F285
• Specificity: Recognizes the p85 subunit of bovine PI-3 Kinase. Species Crossreactivity: Rat, Human, Monkey and Mouse
• Purity/Purification: Purified by Protein G Affinity Chromatography
• Form/Format: Supplied as a liquid in PBS, pH 7.2. Labeled with horseradish peroxidase (HRP).

• Applicable Applications for anti-PIK3R2 antibody: Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blot (WB)
• Immunogen: Balculovirus expressed (recombinant) p85 beta subunit of bovine PI-3 kinase
• Conjugate: HRP
• Preparation and Storage: Store at -20 degree C.

Western Blot (WB)

(Western Blot analysis of HEK293 and HepG2 cell lysate usingMBS607679. Recombinant p85b is included as positive control.)

Product Categories/Family for anti-PIK3R2 antibody

Antibodies; Protein Kinases

References

1. Brehme,M. et al (2009) Proc Natl Acad Sci U S A,. 106: 7414-9 2. Hale, B.G. et al. (2008)J Biol Chem. 283: 1372-80. 3. Ehrhardt, C. et al. (2007) J Virol. 81: 3058-67. 4. Massone, C. et al. (2011) Br J Dermatol. 165: 321-8. 5. Yokoyama, K. et al. (2011) EMBO J. 30: 4739-54.

NCBI and Uniprot Product Information

• NCBI GI #: 27807041
• NCBI GeneID: 282308
• NCBI Accession #: NP_777001.1
• NCBI GenBank Nucleotide #: NM_174576.2
• UniProt Accession #: P23726; Q17QY7
• Molecular Weight: 81,060 Da
• NCBI Official Full Name: phosphatidylinositol 3-kinase regulatory subunit beta
• NCBI Official Synonym Full Names: phosphoinositide-3-kinase, regulatory subunit 2 (beta)
• NCBI Official Symbol: PIK3R2
• NCBI Protein Information: phosphatidylinositol 3-kinase regulatory subunit beta; PI3-kinase p85 subunit beta; PI3-kinase regulatory subunit beta; PI3-kinase subunit p85-beta; PI3K regulatory subunit beta; phosphatidylinositol 3-kinase 85 kDa regulatory subunit beta; phosphoinositi
• UniProt Protein Name: Phosphatidylinositol 3-kinase regulatory subunit beta
• Protein Family: Phosphatidylinositol 3-kinase regulatory
• UniProt Gene Name: PIK3R2
• UniProt Synonym Gene Names: PI3-kinase regulatory subunit beta; PI3K regulatory subunit beta; PtdIns-3-kinase regulatory subunit beta; PI3-kinase subunit p85-beta; PtdIns-3-kinase regulatory subunit p85-beta

Uniprot Description

Regulatory subunit of phosphoinositide-3-kinase (PI3K), a kinase that phosphorylates PtdIns(4,5)P2 (Phosphatidylinositol 4,5-bisphosphate) to generate phosphatidylinositol 3,4,5-trisphosphate (PIP3). PIP3 plays a key role by recruiting PH domain-containing proteins to the membrane, including AKT1 and PDPK1, activating signaling cascades involved in cell growth, survival, proliferation, motility and morphology. Binds to activated (phosphorylated) protein-tyrosine kinases, through its SH2 domain, and acts as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane. Indirectly regulates autophagy. Promotes nuclear translocation of XBP1 in a ER stress- and/or insulin-dependent manner during metabolic overloading in the liver and hence plays a role in glucose tolerance improvement ().