Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Tenomodulin (TNMD) Standard Curve.)
Rat Tenomodulin (TNMD) ELISA Kit | TNMD elisa kit
Rat Tenomodulin (TNMD) ELISA Kit
Gene Names
Tnmd; ChM1L
Reactivity
Rat
Synonyms
Tenomodulin (TNMD); Rat Tenomodulin (TNMD) ELISA Kit; TNMD; BRICD4; CHM1L; TEM; BRICHOS domain containing 4; CHM1-like; chondromodulin I-like; chondromodulin-IB; myodulin; tendin; TNMD elisa kit
Reactivity
Rat
Specificity
Rat Tenomodulin (TNMD) ELISA Kit has high sensitivity and excellent specificity for detection of Rat TNMD. No significant cross-reactivity or interference between Rat TNMD and analogues was observed.
Sequence Length
317
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Tenomodulin (TNMD) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Tenomodulin (TNMD) Standard Curve.)
Related Product Information for TNMD elisa kit
Description: This Rat Tenomodulin (TNMD) ELISA Kit employs a two-site sandwich ELISA to quantitate TNMD in samples. An antibody specific for TNMD has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TNMD present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TNMD is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TNMD bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: By searching an EST database for sequences similar to mouse Tem, followed by RACE of a human fetus cDNA library, Shukunami et al. (2001) cloned TEM. The deduced 317-amino acid protein contains an N-terminal transmembrane domain and a putative antiangiogenic domain with 8 cysteines. TEM shares 96% amino acid identity with mouse Tem, and it shares 65% identity in a 65-amino acid C-terminal stretch with chondromodulin I (CHM1). Northern blot analysis of several mouse tissues detected a 1.4-kb transcript expressed specifically in skeletal muscle. In situ hybridization revealed mouse Tem expression in dense connective tissue, such as epimysium and tendon.
Background: By searching an EST database for sequences similar to mouse Tem, followed by RACE of a human fetus cDNA library, Shukunami et al. (2001) cloned TEM. The deduced 317-amino acid protein contains an N-terminal transmembrane domain and a putative antiangiogenic domain with 8 cysteines. TEM shares 96% amino acid identity with mouse Tem, and it shares 65% identity in a 65-amino acid C-terminal stretch with chondromodulin I (CHM1). Northern blot analysis of several mouse tissues detected a 1.4-kb transcript expressed specifically in skeletal muscle. In situ hybridization revealed mouse Tem expression in dense connective tissue, such as epimysium and tendon.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
tenomodulin
NCBI Official Synonym Full Names
tenomodulin
NCBI Official Symbol
Tnmd
NCBI Official Synonym Symbols
ChM1L
NCBI Protein Information
tenomodulin
UniProt Protein Name
Tenomodulin
Protein Family
UniProt Gene Name
Tnmd
UniProt Synonym Gene Names
Chm1l; TeM; rTeM; ChM1L; rChM1L
UniProt Entry Name
TNMD_RAT
NCBI Description
transmembrane protein; may be an angiogenesis inhibitor; may play a regulatory role in eye and skeletal muscle development [RGD, Feb 2006]
Uniprot Description
TNMD: May be an angiogenesis inhibitor. Belongs to the chondromodulin-1 family.
Protein type: Membrane protein, integral
Cellular Component: cell surface; integral to membrane; nuclear envelope
Biological Process: endothelial cell morphogenesis; negative regulation of angiogenesis; negative regulation of endothelial cell proliferation
Research Articles on TNMD
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Product Notes
The Rat TNMD tnmd (Catalog #AAA9711666) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9711666 ELISA Kit recognizes Rat TNMD. It is sometimes possible for the material contained within the vial of "Tenomodulin (TNMD), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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