Rat Tenomodulin ELISA Kit | Tnmd elisa kit

Rat Tenomodulin ELISA Kit

Cat. Num. AAA762787

• Gene Names: TNMD; TEM; CHM1L; BRICD4
• Reactivity: Rat
• Synonyms: Tenomodulin; Rat Tenomodulin ELISA Kit; TNMD (Tenomodulin)/UNQ771/CHM1L/TeM/hTeM/Tendin/Myodulin/Chondromodulin-1-like protein/Chondromodulin-I-like protein; Tnmd elisa kit

• Reactivity: Rat
• Sequence Length: 317

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 0.156-10ng/ml
• Sensitivity: 0.094ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for Tnmd elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. anti-Rat Tnmd antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Rat Tnmd antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Rat Tnmd amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration ofRat Tnmd can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 156627559
• NCBI GeneID: 64102
• NCBI Accession #: NP_071427.2
• NCBI GenBank Nucleotide #: NM_022144.2
• Molecular Weight: 30,013 Da
• NCBI Official Full Name: tenomodulin
• NCBI Official Synonym Full Names: tenomodulin
• NCBI Official Symbol: TNMD
• NCBI Official Synonym Symbols: TEM; CHM1L; BRICD4
• NCBI Protein Information: tenomodulin
• UniProt Protein Name: Tenomodulin
• Protein Family: Tenomodulin
• UniProt Gene Name: TNMD
• UniProt Synonym Gene Names: CHM1L; TeM; hTeM; ChM1L; hChM1L
• UniProt Entry Name: TNMD_HUMAN

NCBI Description

This gene encodes a protein that is related to chondromodulin-I, which is a cartilage-specific glycoprotein that functions to stimulate chondrocyte growth and to inhibit tube formation of endothelial cells. This protein is also an angiogenesis inhibitor. Genetic variation in this gene is associated with a risk for type 2 diabetes, central obesity and serum levels of systemic immune mediators in a body size-dependent manner. This gene is also a candidate gene for age-related macular degeneration, though a direct link has yet to be demonstrated. [provided by RefSeq, Sep 2009]

Uniprot Description

TNMD: May be an angiogenesis inhibitor. Belongs to the chondromodulin-1 family.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: Xq21.33-q23

Cellular Component: cytoplasm; integral to membrane; nuclear envelope

Molecular Function: protein binding

Biological Process: endothelial cell morphogenesis; negative regulation of angiogenesis; negative regulation of endothelial cell proliferation