Typical Testing Data/Standard Curve (for reference only)
Human Tenomodulin (TNMD) ELISA Kit | TNMD elisa kit
Human Tenomodulin (TNMD) ELISA Kit
Gene Names
TNMD; TEM; CHM1L; BRICD4
Reactivity
Human
Synonyms
Tenomodulin (TNMD); Human Tenomodulin (TNMD) ELISA Kit; TEM; BRICD4; CHM1-LIKE; CHM1L; Myodulin; Tendin; Chondromodulin-1-like protein; TNMD elisa kit
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of TNMD. No significant cross-reactivity or interference between TNMD and analogues was observed.
Sequence Length
254
Samples
Human tissue homogenates, cell lysates and other biological fluids
Assay Type
Quantitative Sandwich
Detection Range
0.312-20ng/mL
Sensitivity
<0.114ng/mL
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level TNMD were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level TNMD were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
Preparation and Storage
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for TNMD elisa kit
Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of TNMD in human tissue homogenates, cell lysates and other biological fluids.
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to TNMD. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to TNMD. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain TNMD, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of TNMD in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to TNMD. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to TNMD. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain TNMD, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of TNMD in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Product Categories/Family for TNMD elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
30,013 Da
NCBI Official Full Name
tenomodulin
NCBI Official Synonym Full Names
tenomodulin
NCBI Official Symbol
TNMD
NCBI Official Synonym Symbols
TEM; CHM1L; BRICD4
NCBI Protein Information
tenomodulin
UniProt Protein Name
Tenomodulin
Protein Family
UniProt Gene Name
TNMD
UniProt Synonym Gene Names
CHM1L; TeM; hTeM; ChM1L; hChM1L
UniProt Entry Name
TNMD_HUMAN
NCBI Description
This gene encodes a protein that is related to chondromodulin-I, which is a cartilage-specific glycoprotein that functions to stimulate chondrocyte growth and to inhibit tube formation of endothelial cells. This protein is also an angiogenesis inhibitor. Genetic variation in this gene is associated with a risk for type 2 diabetes, central obesity and serum levels of systemic immune mediators in a body size-dependent manner. This gene is also a candidate gene for age-related macular degeneration, though a direct link has yet to be demonstrated. [provided by RefSeq, Sep 2009]
Uniprot Description
TNMD: May be an angiogenesis inhibitor. Belongs to the chondromodulin-1 family.
Protein type: Membrane protein, integral
Chromosomal Location of Human Ortholog: Xq21.33-q23
Cellular Component: cytoplasm; integral to membrane; nuclear envelope
Biological Process: negative regulation of angiogenesis; negative regulation of endothelial cell proliferation; endothelial cell morphogenesis
Research Articles on TNMD
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Product Notes
The Human TNMD tnmd (Catalog #AAA2022191) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2022191 ELISA Kit recognizes Human TNMD. It is sometimes possible for the material contained within the vial of "Tenomodulin (TNMD), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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