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Typical Testing Data/Standard Curve (for reference only) (Fig.1. Rat Synapsin-1 (SYN1) Standard Curve.)

Rat Synapsin-1 (SYN1) ELISA Kit | SYN1 elisa kit

Rat Synapsin-1 (SYN1) ELISA Kit

Reactivity
Rat
Synonyms
Synapsin-1 (SYN1); Rat Synapsin-1 (SYN1) ELISA Kit; SYN1; RP1-230G1.2; SYN1a; SYN1b; SYNI; brain protein 4.1; SYN1 elisa kit
Ordering
For Research Use Only!
Reactivity
Rat
Specificity
Rat Synapsin-1 (SYN1) ELISA Kit has high sensitivity and excellent specificity for detection of Rat SYN1. No significant cross-reactivity or interference between Rat SYN1 and analogues was observed.
Sequence Length
668
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

(Fig.1. Rat Synapsin-1 (SYN1) Standard Curve.)

Typical Testing Data/Standard Curve (for reference only) (Fig.1. Rat Synapsin-1 (SYN1) Standard Curve.)
Related Product Information for SYN1 elisa kit
Description: This Rat Synapsin-1 (SYN1) ELISA Kit employs a two-site sandwich ELISA to quantitate SYN1 in samples. An antibody specific for SYN1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SYN1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SYN1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SYN1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Background: The synapsin I protein is a member of the synapsin family that are neuronal phosphoproteins which associate with the cytoplasmic surface of synaptic vesicles. Family members are characterized by common protein domains, and they are implicated in synaptogenesis and the modulation of neurotransmitter release, suggesting a potential role in several neuropsychiatric diseases.The phosphoprotein plays a role in regulation of axonogenesis and synaptogenesis. The protein serves as a substrate for several different protein kinases and phosphorylation may function in the regulation of this protein in the nerve terminal.Synapsin I is found in two isoforms of the protein, Synapsin Ia and Synapsin Ib, with Synapsin Ib being a slightly shorter version of the protein.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
synapsin-1 isoform b
NCBI Official Synonym Full Names
synapsin I
NCBI Official Symbol
Syn1
NCBI Protein Information
synapsin-1
UniProt Protein Name
Synapsin-1
Protein Family
UniProt Gene Name
Syn1
UniProt Entry Name
SYN1_RAT

NCBI Description

may act as an anchoring protein by linking the vesicle phospholipid layer and the cytoskeleton in neuronal presynaptic terminals; may play a role in neurotransmitter release [RGD, Feb 2006]

Uniprot Description

SYN1: neuronal phosphoprotein which associates with the cytoplasmic surface of synaptic vesicles and binds to the cytoskeleton. May function in the regulation of neurotransmitter release and of axonogenesis and synaptogenesis. Mutations may be associated with X-linked disorders with primary neuronal degeneration such as Rett syndrome. Two differentially spiced isoforms have been reported.

Protein type: Vesicle

Cellular Component: Golgi apparatus; synaptic vesicle membrane; cell; dendrite; postsynaptic density; intracellular organelle; terminal button; synaptonemal complex; cytosol; synaptic vesicle; axon; presynaptic active zone; synapse; cell junction

Molecular Function: protein binding; actin binding; calcium-dependent protein binding; catalytic activity; protein kinase binding; ATP binding

Biological Process: metabolic process; neurotransmitter secretion

Research Articles on SYN1

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Product Notes

The Rat SYN1 syn1 (Catalog #AAA9711754) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9711754 ELISA Kit recognizes Rat SYN1. It is sometimes possible for the material contained within the vial of "Synapsin-1 (SYN1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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