Human Synapsin I (SYN1) ELISA Kit | SYN1 elisa kit

Human Synapsin I (SYN1) ELISA Kit

Cat. Num. AAA2088172

• Gene Names: SYN1; SYNI; SYN1a; SYN1b
• Reactivity: Human
• Synonyms: Synapsin I (SYN1); Human Synapsin I (SYN1) ELISA Kit; SYN1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Synapsin I (SYN1).; No significant cross-reactivity or interference between Synapsin I (SYN1) and analogues was observed.
• Sequence Length: 705

• Assay Type: Double-antibody Sandwich
• Samples: Tissue homogenates, Cell lysates, Cell culture supernates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.071ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Synapsin I (SYN1) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Synapsin I (SYN1) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for SYN1 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Synapsin I (SYN1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Synapsin I (SYN1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Synapsin I (SYN1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Synapsin I (SYN1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for SYN1 elisa kit

Neuroscience

NCBI and Uniprot Product Information

• NCBI GI #: 19924099
• NCBI GeneID: 6853
• NCBI Accession #: NP_008881.2
• NCBI GenBank Nucleotide #: NM_006950.3
• UniProt Accession #: P17600; O75825; Q5H9A9; B1AJQ1
• Molecular Weight: 70,033 Da
• NCBI Official Full Name: synapsin-1 isoform Ia
• NCBI Official Synonym Full Names: synapsin I
• NCBI Official Symbol: SYN1
• NCBI Official Synonym Symbols: SYNI; SYN1a; SYN1b
• NCBI Protein Information: synapsin-1
• UniProt Protein Name: Synapsin-1
• Protein Family: Synapsin
• UniProt Gene Name: SYN1

NCBI Description

This gene is a member of the synapsin gene family. Synapsins encode neuronal phosphoproteins which associate with the cytoplasmic surface of synaptic vesicles. Family members are characterized by common protein domains, and they are implicated in synaptogenesis and the modulation of neurotransmitter release, suggesting a potential role in several neuropsychiatric diseases. This member of the synapsin family plays a role in regulation of axonogenesis and synaptogenesis. The protein encoded serves as a substrate for several different protein kinases and phosphorylation may function in the regulation of this protein in the nerve terminal. Mutations in this gene may be associated with X-linked disorders with primary neuronal degeneration such as Rett syndrome. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]

Uniprot Description

SYN1: neuronal phosphoprotein which associates with the cytoplasmic surface of synaptic vesicles and binds to the cytoskeleton. May function in the regulation of neurotransmitter release and of axonogenesis and synaptogenesis. Mutations may be associated with X-linked disorders with primary neuronal degeneration such as Rett syndrome. Two differentially spiced isoforms have been reported.

Protein type: Vesicle

Chromosomal Location of Human Ortholog: Xp11.3-p11.23

Cellular Component: synaptic vesicle

Molecular Function: ATP binding; protein binding; protein kinase binding; transporter activity

Biological Process: regulation of neurotransmitter secretion; synaptic transmission

Disease: Epilepsy, X-linked, With Variable Learning Disabilities And Behavior Disorders