Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Solute carrier family 2, facilitated glucose transporter member 1 (SLC2A1) Standard Curve.)
Rat Solute Carrier Family 2, Facilitated Glucose Transporter Member 1 (SLC2A1) ELISA Kit | SLC2A1 elisa kit
Rat Solute Carrier Family 2, Facilitated Glucose Transporter Member 1 (SLC2A1) ELISA Kit
Gene Names
SLC2A1; GLUT1
Reactivity
Rat
Synonyms
Solute Carrier Family 2; Facilitated Glucose Transporter Member 1 (SLC2A1); Rat Solute Carrier Family 2; Facilitated Glucose Transporter Member 1 (SLC2A1) ELISA Kit; SLC2A1; DYT17; DYT18; GLUT; GLUT1; MGC141895; MGC141896; PED; SLC2A1 elisa kit
Reactivity
Rat
Specificity
Rat Solute carrier family 2, facilitated glucose transporter member 1 (SLC2A1) ELISA Kit has high sensitivity and excellent specificity for detection of Rat SLC2A1. No significant cross-reactivity or interference between Rat SLC2A1 and analogues was observed.
Sequence Length
492
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Solute carrier family 2, facilitated glucose transporter member 1 (SLC2A1) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Solute carrier family 2, facilitated glucose transporter member 1 (SLC2A1) Standard Curve.)
Related Product Information for SLC2A1 elisa kit
Description: This Rat Solute carrier family 2, facilitated glucose transporter member 1 (SLC2A1) ELISA Kit employs a two-site sandwich ELISA to quantitate SLC2A1 in samples. An antibody specific for SLC2A1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SLC2A1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SLC2A1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SLC2A1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: GLUT1 facilitates the transport of glucose across the plasma membranes of mammalian cells.GLUT1 is responsible for the low-level of basal glucose uptake required to sustain respiration in all cells. Expression levels of GLUT1 in cell membranes are increased by reduced glucose levels and decreased by increased glucose levels. GLUT1 is also a major receptor for take-up of Vitamin C as well as glucose, especially in non vitamin C producing mammals as part of an adaptation to compensate by participating in a Vitamin C recycling process. In mammals that do produce Vitamin C, GLUT4 is often expressed instead of GLUT1.GLUT1 behaves as a Michaelis-Menten enzyme and contains 12 membrane-spanning alpha helices, each containing 20 amino acid residues.
Background: GLUT1 facilitates the transport of glucose across the plasma membranes of mammalian cells.GLUT1 is responsible for the low-level of basal glucose uptake required to sustain respiration in all cells. Expression levels of GLUT1 in cell membranes are increased by reduced glucose levels and decreased by increased glucose levels. GLUT1 is also a major receptor for take-up of Vitamin C as well as glucose, especially in non vitamin C producing mammals as part of an adaptation to compensate by participating in a Vitamin C recycling process. In mammals that do produce Vitamin C, GLUT4 is often expressed instead of GLUT1.GLUT1 behaves as a Michaelis-Menten enzyme and contains 12 membrane-spanning alpha helices, each containing 20 amino acid residues.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
solute carrier family 2, facilitated glucose transporter member 1
NCBI Official Symbol
SLC2A1
NCBI Official Synonym Symbols
GLUT1
NCBI Protein Information
solute carrier family 2, facilitated glucose transporter member 1
UniProt Protein Name
Solute carrier family 2, facilitated glucose transporter member 1
Protein Family
UniProt Gene Name
SLC2A1
UniProt Synonym Gene Names
GLUT1; GLUT-1
UniProt Entry Name
GTR1_RABIT
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Product Notes
The Rat SLC2A1 slc2a1 (Catalog #AAA9711871) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9711871 ELISA Kit recognizes Rat SLC2A1. It is sometimes possible for the material contained within the vial of "Solute Carrier Family 2, Facilitated Glucose Transporter Member 1 (SLC2A1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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