Rabbit anti-Human, Rat SLC2A1 Polyclonal Antibody | anti-SLC2A1 antibody

SLC2A1 polyclonal antibody

Cat. Num. AAA3000037

• Gene Names: SLC2A1; PED; DYT9; GLUT; DYT17; DYT18; EIG12; GLUT1; HTLVR; GLUT1DS
• Reactivity: Human, Rat
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: SLC2A1; Polyclonal Antibody; SLC2A1 polyclonal antibody; Solute carrier family 2; facilitated glucose transporter member 1; Glucose transporter type 1; erythrocyte/brain; GLUT-1; anti-SLC2A1 antibody

• Host: Rabbit
• Reactivity: Human, Rat
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: SLC2A1 polyclonal antibody detects endogenous levels of SLC2A1 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.4.

• Applicable Applications for anti-SLC2A1 antibody: Western Blot (WB)
• Application Notes: WB: 1:1000~1:2000
• Immunogen: Synthetic peptide, corresponding to Human SLC2A1.
• Preparation and Storage: Store at 4 degee C for short term. Aliquot and store at -20 degee C long term. Avoid freeze-thaw cycles.

Western Blot (WB)

(Western blot (WB) analysis of SLC2A1 polyclonal antibody at 1:500 dilutionLane1:PC12 whole cell lysate(40ug)Lane2:SGC7901 whole cell lysate(40ug)Lane3:EC9706 whole cell lysate(40ug))

Testing Data

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Related Product Information for anti-SLC2A1 antibody

Background: Glucose transporter 1 (Glut1, SLC2A1) is a widely expressed transport protein that displays a broad range of substrate specificity in transporting a number of different aldose sugars as well as an oxidized form of vitamin C into cells. Glut1 is responsible for the basal-level uptake of glucose from the blood through facilitated diffusion. Research studies show that Glut1 and the transcription factor HIF-1alpha mediate the regulation of glycolysis by O-GlcNAcylation in cancer cells. Additional studies demonstrate that Glut1 is required for CD4 T cell activation and is critical for the expansion and survival of T effector (Teff) cells. Mutations in the corresponding SLC2A1 gene cause GLUT1 deficiency syndromes (GLUT1DS1, GLUT1DS2), a pair of neurologic disorders characterized by delayed development, seizures, spasticity, paroxysmal exercise-induced dyskinesia, and acquired microcephaly. Two other neurologic disorders-dystonia-9 (DYT9) and susceptibility to idiopathic generalized epilepsy 12 (EIG12)-are also caused by mutations in the SLC2A1 gene.

NCBI and Uniprot Product Information

• NCBI GI #: 166795299
• NCBI GeneID: 6513
• NCBI Accession #: NP_006507.2
• NCBI GenBank Nucleotide #: NM_006516.2
• UniProt Accession #: P11166; O75535; Q147X2; A8K9S6; B2R620; D3DPX0
• Molecular Weight: 54,084 Da
• NCBI Official Full Name: solute carrier family 2, facilitated glucose transporter member 1
• NCBI Official Synonym Full Names: solute carrier family 2 (facilitated glucose transporter), member 1
• NCBI Official Symbol: SLC2A1
• NCBI Official Synonym Symbols: PED; DYT9; GLUT; DYT17; DYT18; EIG12; GLUT1; HTLVR; GLUT1DS
• NCBI Protein Information: solute carrier family 2, facilitated glucose transporter member 1; GLUT-1; hepG2 glucose transporter; glucose transporter type 1, erythrocyte/brain; human T-cell leukemia virus (I and II) receptor
• UniProt Protein Name: Solute carrier family 2, facilitated glucose transporter member 1
• Protein Family: Solute carrier family
• UniProt Gene Name: SLC2A1
• UniProt Synonym Gene Names: GLUT1; GLUT-1
• UniProt Entry Name: GTR1_HUMAN

NCBI Description

This gene encodes a major glucose transporter in the mammalian blood-brain barrier. The encoded protein is found primarily in the cell membrane and on the cell surface, where it can also function as a receptor for human T-cell leukemia virus (HTLV) I and II. Mutations in this gene have been found in a family with paroxysmal exertion-induced dyskinesia. [provided by RefSeq, Apr 2013]

Uniprot Description

GLUT1: an integral membrane protein that plays an important role in the glycolytic pathway by serving as a uniporter for glucose. One of 13 members of the human equilibrative glucose transport protein family. Transports a wide range of aldoses, including both pentoses and hexoses, and dehydroascorbic acid. Shown to transport water against an osmotic gradient. A receptor for the Human T-cell Leukemia virus (HTLV). Plays a role in the constitutive or basal uptake of glucose. Expressed at highest levels in proliferating cells of the early developing embryo, cells forming the blood tissue barriers, in human erythrocytes, astrocytes and in cardiac muscle. GLUT1 and GLUT3 are both essential for normal embryonic development. Is practically the only member of the GLUT family expressed on human red blood cells, where it comprises 10 - 20% of the integral membrane protein content. Several glycolytic proteins including the transporters GLUT1 and GLUT3, as well as multiple enzymes including HK2, PFKL, LDHA, ALDOA, ALDOC, PGK1, ENO1, PKM2, CA9 and PFKFB3 are induced in cancer cells by HIF-1 alpha. Polyps from Peutz-Jeghers patients exhibit up-regulated mTORC1 signaling, HIF-1alpha, and GLUT1 levels. Defects in GLUT1 are the cause of autosomal dominant GLUT1 deficiency syndrome, a blood-brain barrier glucose transport defect characterized by infantile seizures, delayed development, and acquired microcephaly. Defects also cause dystonia type 18, an exercise-induced paroxysmal dystonia/dyskinesia. Cytochalasin B binds to its inner surface, inhibiting its glucose transport activity with an IC50 of 0.44 uM.

Protein type: Membrane protein, integral; Membrane protein, multi-pass; Transporter, SLC family; Transporter

Chromosomal Location of Human Ortholog: 1p34.2

Cellular Component: cortical actin cytoskeleton; membrane; basolateral plasma membrane; integral to plasma membrane; melanosome; plasma membrane; female pronucleus; midbody; caveola; intercellular junction; cytosol

Molecular Function: identical protein binding; D-glucose transmembrane transporter activity; xenobiotic transporter activity; protein binding; protein self-association; dehydroascorbic acid transporter activity; glucose transmembrane transporter activity; kinase binding

Biological Process: cellular response to glucose starvation; vitamin metabolic process; hexose transport; carbohydrate metabolic process; L-ascorbic acid metabolic process; energy reserve metabolic process; pathogenesis; xenobiotic transport; protein complex assembly; glucose transport; response to osmotic stress; transmembrane transport; regulation of insulin secretion; water-soluble vitamin metabolic process

Disease: Epilepsy, Idiopathic Generalized, Susceptibility To, 12; Dystonia 9; Glut1 Deficiency Syndrome 1; Glut1 Deficiency Syndrome 2