Immunofluorescence (IF)
(Immunofluorescence staining of fixed human peripheral blood leukocytes with anti-IL-8RB antibody HC2 (MBS488771) Immunofluorescence analysis of paraformaldehyde fixed human peripheral blood leukocytes on Shi-fix coverslips stained with the chimeric rabbit IgG version of HC2 (MBS488771) at 10ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom MBS488771, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (MBS488140) followed by staining with Alexa Fluor 488 secondary antibody.)
Mouse anti-Human IL-8RB Monoclonal Antibody | anti-IL-8RB antibody
Anti-IL-8RB [HC2]
Immunofluorescence (IF)
(Immunofluorescence staining of fixed human peripheral blood leukocytes with anti-IL-8RB antibody HC2 (MBS488771) Immunofluorescence analysis of paraformaldehyde fixed human peripheral blood leukocytes on Shi-fix coverslips stained with the chimeric rabbit IgG version of HC2 (MBS488771) at 10ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom MBS488771, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (MBS488140) followed by staining with Alexa Fluor 488 secondary antibody.)
Immunofluorescence (IF)
(Immunofluorescence staining of fixed human peripheral blood leukocytes with anti-IL-8RB antibody HC2 (MBS488771) Immunofluorescence analysis of paraformaldehyde fixed human peripheral blood leukocytes on Shi-fix coverslips stained with the chimeric rabbit IgG version of HC2 (MBS488771) at 10ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom MBS488771, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (MBS488140) followed by staining with Alexa Fluor 488 secondary antibody.)
This antibody was raised by immunizing mice with human IL-8 (interleukin-8) receptor, type B.
Note on publication: This article describes the generation and characterization of HC2 antibody.
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a member of the G-protein-coupled receptor family. This protein is a receptor for interleukin 8 (IL8). It binds to IL8 with high affinity, and transduces the signal through a G-protein activated second messenger system. This receptor also binds to chemokine (C-X-C motif) ligand 1 (CXCL1/MGSA), a protein with melanoma growth stimulating activity, and has been shown to be a major component required for serum-dependent melanoma cell growth. This receptor mediates neutrophil migration to sites of inflammation. The angiogenic effects of IL8 in intestinal microvascular endothelial cells are found to be mediated by this receptor. Knockout studies in mice suggested that this receptor controls the positioning of oligodendrocyte precursors in developing spinal cord by arresting their migration. This gene, IL8RA, a gene encoding another high affinity IL8 receptor, as well as IL8RBP, a pseudogene of IL8RB, form a gene cluster in a region mapped to chromosome 2q33-q36. Alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Nov 2009]
Uniprot Description
IL-8R B: Receptor for interleukin-8 which is a powerful neutrophil chemotactic factor. Binding of IL-8 to the receptor causes activation of neutrophils. This response is mediated via a G-protein that activates a phosphatidylinositol-calcium second messenger system. Binds to IL-8 with high affinity. Also binds with high affinity to CXCL3, GRO/MGSA and NAP-2. Belongs to the G-protein coupled receptor 1 family.
Protein type: Receptor, GPCR; Membrane protein, integral; GPCR, family 1; Membrane protein, multi-pass; Receptor, cytokine
Chromosomal Location of Human Ortholog: 2q35
Cellular Component: cell surface; mast cell granule; membrane; integral to plasma membrane; plasma membrane; intracellular
Molecular Function: signal transducer activity; protein binding; interleukin-8 receptor activity; interleukin-8 binding; C-X-C chemokine receptor activity
Biological Process: neutrophil chemotaxis; neutrophil activation; signal transduction; chemotaxis; positive regulation of vascular permeability; negative regulation of neutrophil apoptosis; positive regulation of angiogenesis; elevation of cytosolic calcium ion concentration; cell surface receptor linked signal transduction; receptor internalization; midbrain development; positive regulation of cell proliferation; cellular defense response; dendritic cell chemotaxis; G-protein signaling, coupled to IP3 second messenger (phospholipase C activating); inflammatory response; acute inflammatory response to antigenic stimulus
Research Articles on IL-8RB
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Product Notes
The IL-8RB cxcr2 (Catalog #AAA488770) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-IL-8RB [HC2] reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's IL-8RB can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS). This antibody can be used in the detection and analysis of IL-8RB via flow cytometry (Zahn et al., 1997). Researchers should empirically determine the suitability of the IL-8RB cxcr2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "IL-8RB, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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