Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Serine/threonine-protein kinase TAO1 (TAOK1) Standard Curve.)
Rat Serine/Threonine-Protein Kinase TAO1 (TAOK1) ELISA Kit | TAOK1 elisa kit
Rat Serine/Threonine-Protein Kinase TAO1 (TAOK1) ELISA Kit
Reactivity
Rat
Synonyms
Serine/Threonine-Protein Kinase TAO1 (TAOK1); Rat Serine/Threonine-Protein Kinase TAO1 (TAOK1) ELISA Kit; TAOK1; FLJ14314; KIAA1361; MAP3K16; MARKK; PSK2; TAO1; STE20-like kinase; microtubule affinity regulating kinase kinase; serine/threonine kinase TAO1; serine/threonine protein kinase TAO1 homolog; TAOK1 elisa kit
Reactivity
Rat
Specificity
Rat Serine/threonine-protein kinase TAO1 (TAOK1) ELISA Kit has high sensitivity and excellent specificity for detection of Rat TAOK1. No significant cross-reactivity or interference between Rat TAOK1 and analogues was observed.
Sequence Length
1001
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Serine/threonine-protein kinase TAO1 (TAOK1) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Serine/threonine-protein kinase TAO1 (TAOK1) Standard Curve.)
Related Product Information for TAOK1 elisa kit
Description: This Rat Serine/threonine-protein kinase TAO1 (TAOK1) ELISA Kit employs a two-site sandwich ELISA to quantitate TAOK1 in samples. An antibody specific for TAOK1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TAOK1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TAOK1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TAOK1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Serine/threonine-protein kinase involved in various processes such as p38/MAPK14 stress-activated MAPK cascade, DNA damage response and regulation of cytoskeleton stability. Phosphorylates MAP2K3, MAP2K6 and MARK2. Acts as an activator of the p38/MAPK14 stress-activated MAPK cascade by mediating phosphorylation and subsequent activation of the upstream MAP2K3 and MAP2K6 kinases. Involved in G-protein coupled receptor signaling to p38/MAPK14. In response to DNA damage, involved in the G2/M transition DNA damage checkpoint by activating the p38/MAPK14 stress-activated MAPK cascade, probably by mediating phosphorylation of MAP2K3 and MAP2K6. Acts as a regulator of cytoskeleton stability by phosphorylating 'Thr-208' of MARK2, leading to activate MARK2 kinase activity and subsequent phosphorylation and detachment of MAPT/TAU from microtubules. Also acts as a regulator of apoptosis: regulates apoptotic morphological changes, including cell contraction, membrane blebbing and apoptotic bodies formation via activation of the MAPK8/JNK cascade.
Background: Serine/threonine-protein kinase involved in various processes such as p38/MAPK14 stress-activated MAPK cascade, DNA damage response and regulation of cytoskeleton stability. Phosphorylates MAP2K3, MAP2K6 and MARK2. Acts as an activator of the p38/MAPK14 stress-activated MAPK cascade by mediating phosphorylation and subsequent activation of the upstream MAP2K3 and MAP2K6 kinases. Involved in G-protein coupled receptor signaling to p38/MAPK14. In response to DNA damage, involved in the G2/M transition DNA damage checkpoint by activating the p38/MAPK14 stress-activated MAPK cascade, probably by mediating phosphorylation of MAP2K3 and MAP2K6. Acts as a regulator of cytoskeleton stability by phosphorylating 'Thr-208' of MARK2, leading to activate MARK2 kinase activity and subsequent phosphorylation and detachment of MAPT/TAU from microtubules. Also acts as a regulator of apoptosis: regulates apoptotic morphological changes, including cell contraction, membrane blebbing and apoptotic bodies formation via activation of the MAPK8/JNK cascade.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
serine/threonine-protein kinase TAO1
NCBI Official Synonym Full Names
TAO kinase 1
NCBI Official Symbol
Taok1
NCBI Protein Information
serine/threonine-protein kinase TAO1
UniProt Protein Name
Serine/threonine-protein kinase TAO1
Protein Family
UniProt Gene Name
Taok1
UniProt Synonym Gene Names
Tao1
UniProt Entry Name
TAOK1_RAT
NCBI Description
protein kinase; involved in regulation of the p38-containing stress-responsive MAP kinase pathway [RGD, Feb 2006]
Research Articles on TAOK1
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Product Notes
The Rat TAOK1 taok1 (Catalog #AAA9711795) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9711795 ELISA Kit recognizes Rat TAOK1. It is sometimes possible for the material contained within the vial of "Serine/Threonine-Protein Kinase TAO1 (TAOK1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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