Rabbit anti-Human TAO Kinase 1 Polyclonal Antibody | anti-TAOK1 antibody

TAO Kinase 1 (Thousand and One Amino Acid Protein Kinase 1, TAOK1, hTAOK1, TAO1, Kinase from Chicken Homolog B, KFC-B, hKFC-B, KIAA1361, MAP3K16, MARK Kinase, MARKK, Prostate-derived Sterile 20-like Kinase 2, Prostate-derived STE20-like Kinase 2, PSK2, PS

Cat. Num. AAA626998

• Gene Names: TAOK1; PSK2; TAO1; KFC-B; MARKK; hKFC-B; MAP3K16; FLJ14314; KIAA1361
• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: Affinity Purified; Purified by Protein G affinity chromatography.
• Synonyms: TAO Kinase 1; Polyclonal Antibody; TAO Kinase 1 (Thousand and One Amino Acid Protein Kinase 1; TAOK1; hTAOK1; TAO1; Kinase from Chicken Homolog B; KFC-B; hKFC-B; KIAA1361; MAP3K16; MARK Kinase; MARKK; Prostate-derived Sterile 20-like Kinase 2; Prostate-derived STE20-like Kinase 2; PSK2; PS; Anti -TAO Kinase 1 (Thousand and One Amino Acid Protein Kinase 1; anti-TAOK1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human TAOK1.
• Purity/Purification: Affinity Purified; Purified by Protein G affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, 0.09% sodium azide.

• Applicable Applications for anti-TAOK1 antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in ELISA and Western Blot.; Dilution: ELISA: 1:1,000; Western Blot: 1:100-1:500
• Immunogen: Synthetic peptide selected from the center region of human TAOK1 (KLH).
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of anti-TAOK1 Pab (AP7085c) in mouse brain tissue lysate (35ug/lane). TAOK1(arrow) was detected using the purified Pab.)

Related Product Information for anti-TAOK1 antibody

TAOK1 is an upstream activator of Mark. TAOK1 phosphorylated Mark on a threonine within its activation loop. In brain, TAOK1 also phosphorylated a fraction of Mark on a nearby serine, and this phosphorylation inhibited Mark activity. In cells, TAOK1 activity enhanced microtubule dynamics through activation of Mark and led to phosphorylation and detachment of microtubule-associated proteins from microtubules. TAOK1 also activated JNK in vitro. Overexpression of TAOK1 in a human nonsmall cell lung carcinoma cell line induced apoptotic morphologic changes, including cell contraction, membrane blebbing, and apoptotic body formation. Apoptotic stimuli increased the catalytic activity of endogenous TAOK1 and JNK, and dominant-negative JNK or JNK inhibition blocked the apoptotic morphologic responses to TAOK1. TAOK1 also stimulated cleavage and activation of ROCK1 by caspases, leading to cell contraction and membrane blebbing. TAOK1 was itself a substrate for caspase-3. TAOK1 is indeed involved in the execution phase of apoptosis.

Product Categories/Family for anti-TAOK1 antibody

Antibodies; Abs to Protein Kinases

NCBI and Uniprot Product Information

• NCBI GI #: 219519599
• NCBI GeneID: 57551
• UniProt Accession #: Q7L7X3; Q96L75; Q9H2K7; Q9H7S5; Q9P2I6; A2RUT8
• Molecular Weight: 116,070 Da
• NCBI Official Full Name: TAOK1 protein
• NCBI Official Synonym Full Names: TAO kinase 1
• NCBI Official Symbol: TAOK1
• NCBI Official Synonym Symbols: PSK2; TAO1; KFC-B; MARKK; hKFC-B; MAP3K16; FLJ14314; KIAA1361
• NCBI Protein Information: serine/threonine-protein kinase TAO1; STE20-like kinase PSK2; kinase from chicken homolog B; thousand and one amino acid protein 1; serine/threonine protein kinase TAO1 homolog; microtubule affinity regulating kinase kinase
• UniProt Protein Name: Serine/threonine-protein kinase TAO1
• Protein Family: Serine/threonine-protein kinase
• UniProt Gene Name: TAOK1
• UniProt Synonym Gene Names: KIAA1361; MAP3K16
• UniProt Entry Name: TAOK1_HUMAN

Uniprot Description

TAO1: Serine/threonine-protein kinase involved in various processes such as p38/MAPK14 stress-activated MAPK cascade, DNA damage response and regulation of cytoskeleton stability. Phosphorylates MAP2K3, MAP2K6 and MARK2. Acts as an activator of the p38/MAPK14 stress-activated MAPK cascade by mediating phosphorylation and subsequent activation of the upstream MAP2K3 and MAP2K6 kinases. Involved in G-protein coupled receptor signaling to p38/MAPK14. In response to DNA damage, involved in the G2/M transition DNA damage checkpoint by activating the p38/MAPK14 stress-activated MAPK cascade, probably by mediating phosphorylation of MAP2K3 and MAP2K6. Acts as a regulator of cytoskeleton stability by phosphorylating 'Thr-208' of MARK2, leading to activate MARK2 kinase activity and subsequent phosphorylation and detachment of MAPT/TAU from microtubules. Also acts as a regulator of apoptosis: regulates apoptotic morphological changes, including cell contraction, membrane blebbing and apoptotic bodies formation via activation of the MAPK8/JNK cascade. Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Protein kinase, STE; Protein kinase, Ser/Thr (non-receptor); Kinase, protein; EC 2.7.11.1; STE group; STE20 family; TAO subfamily

Chromosomal Location of Human Ortholog: 17q11.2

Cellular Component: cytosol

Molecular Function: transferase activity; protein serine/threonine kinase activity; protein binding; MAP kinase kinase kinase activity; kinase activity; ATP binding; protein kinase activity

Biological Process: activation of MAPKK activity; spindle checkpoint; MAPKKK cascade; positive regulation of JNK cascade; mitotic cell cycle; DNA repair; G2/M transition DNA damage checkpoint; response to DNA damage stimulus; protein amino acid phosphorylation; positive regulation of stress-activated MAPK cascade; regulation of cytoskeleton organization and biogenesis