Typical Testing Data/Standard Curve (for reference only)
Rat SEMA6A ELISA Kit | SEMA6A elisa kit
Rat SEMA6A PicoKine ELISA Kit
Reactivity
Rat
Synonyms
SEMA6A; Rat SEMA6A PicoKine ELISA Kit; Rat SEMA6A; Semaphorin 6A; Sema6a; SEMA6A elisa kit
Reactivity
Rat
Specificity
Natural and recombinant Rat Sema6a
Samples
Cell culture supernates, serum and plasma (heparin, EDTA, citrate)
Detection Range
62.5pg/ml-4000pg/ml
Sensitivity
<50pg/ml
Intra-assay Precision
Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-assay Precision
Inter-Assay Precision (Precision across assays):Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Preparation and Storage
Store at 4 degree C for 6 months. Store at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for SEMA6A elisa kit
Principle of the Assay: The Picokine™ Rat Sema6a Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Rat Sema6a with a 96-well strip plate that is pre-coated with antibody specific for Sema6a. The detection antibody is a biotinylated antibody specific for Sema6a. The capture antibody is a monoclonal antibody from mouse, the detection antibody is a biotinylated polyclonal antibody from goat. The kit contains recombinant Rat Sema6a with immunogen: Expression system for standard: NSO, Immunogen sequence: G19-T649. The kit is analytically validated with ready to use reagents. To measure Rat Sema6a, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is substrate to HRP and will be catalyzed to produce a blue color product, which changes into yellow after adding acidic stop solution. The density of the yellow product is linearly proportional to Rat Sema6a in the sample. Read the density of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Rat Sema6a in the sample.!!Background/Introduction: Semaphorin-6A is a protein that in humans is encoded by the SEMA6A gene. This gene is mapped to 18q11. Semaphorin 6A is a transmembrane protein belonging to the semaphorin family. It functions as an axon guidance signal.
NCBI and Uniprot Product Information
NCBI GeneID
UniProt Accession #
NCBI Official Synonym Full Names
semaphorin 6A
NCBI Official Symbol
Sema6a
NCBI Protein Information
semaphorin-6A
Protein Family
NCBI Description
mouse homolog is a transmembrane protein that binds to the protein EVL (Ena/VASP-like protein) [RGD, Feb 2006]
Research Articles on SEMA6A
Similar Products
Product Notes
The Rat SEMA6A (Catalog #AAA1752662) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA1752662 ELISA Kit recognizes Rat SEMA6A. It is sometimes possible for the material contained within the vial of "SEMA6A, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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