Typical Testing Data/Standard Curve (for reference only)
Rat Cadherin-4/R-Cadherin ELISA Kit | CDH4 elisa kit
Rat Cadherin-4/R-Cadherin PicoKine ELISA Kit
Gene Names
Cdh4; Cdh4l
Reactivity
Rat
Synonyms
Cadherin-4/R-Cadherin; Rat Cadherin-4/R-Cadherin PicoKine ELISA Kit; Rat Cadherin-4/R-Cadherin; cadherin 4; Cadherin-4; Retinal cadherin; R-CAD; R-cadherin; Cdh4; CDH4 elisa kit
Reactivity
Rat
Specificity
Natural and recombinant Rat Cdh4
Sequence Length
296
Samples
Cell culture supernates, serum and plasma (heparin, EDTA, citrate)
Detection Range
156pg/ml-10000pg/ml
Sensitivity
<50pg/ml
Intra-assay Precision
Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-assay Precision
Inter-Assay Precision (Precision across assays):Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Preparation and Storage
Store at 4 degree C for 6 months. Store at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for CDH4 elisa kit
Principle of the Assay: The Picokine™ Rat Cdh4 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Rat Cdh4 with a 96-well strip plate that is pre-coated with antibody specific for Cdh4. The detection antibody is a biotinylated antibody specific for Cdh4. The capture antibody is a monoclonal antibody from mouse, the detection antibody is a biotinylated polyclonal antibody from goat. The kit contains recombinant Rat Cdh4 with immunogen: Expression system for standard: NSO, Immunogen sequence: D167-A731. The kit is analytically validated with ready to use reagents. To measure Rat Cdh4, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is substrate to HRP and will be catalyzed to produce a blue color product, which changes into yellow after adding acidic stop solution. The density of the yellow product is linearly proportional to Rat Cdh4 in the sample. Read the density of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Rat Cdh4 in the sample.!!Background/Introduction: Cadherin-4 is a protein that in humans is encoded by the CDH4 gene. It is mapped to 3q43. This gene encodes a member of the cadherin family of calcium-dependent glycoproteins that mediate cell adhesion and regulate many morphogenetic events during development. The encoded preproprotein is further processed to generate a mature protein. The encoded protein is involved in retinal angiogenesis during development where it plays a crucial role in the endothelial-astrocyte interactions. Alternative splicing results in multiple transcript variants encoding different isoforms.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
NCBI Official Full Name
Cadherin-4
NCBI Official Synonym Full Names
cadherin 4
NCBI Official Symbol
Cdh4
NCBI Official Synonym Symbols
Cdh4l
NCBI Protein Information
cadherin-4; LOW QUALITY PROTEIN: cadherin-4
UniProt Protein Name
Cadherin-4
UniProt Gene Name
Cdh4
UniProt Synonym Gene Names
R-CAD; R-cadherin
UniProt Entry Name
CADH4_RAT
NCBI Description
mouse homolog is a calcium dependent cell-cell adhesion molecule that plays a role in retinal endothelial cell guidance [RGD, Feb 2006]
Uniprot Description
CDH4: Cadherins are calcium dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. May play an important role in retinal development. Expressed mainly in brain but also found in other tissues.
Protein type: Motility/polarity/chemotaxis; Cell adhesion; Membrane protein, integral
Cellular Component: integral to plasma membrane; plasma membrane; integral to membrane
Molecular Function: calcium ion binding
Biological Process: heterophilic cell adhesion; axon guidance; positive regulation of axon extension; homophilic cell adhesion
Research Articles on CDH4
Similar Products
Product Notes
The Rat CDH4 cdh4 (Catalog #AAA1752700) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA1752700 ELISA Kit recognizes Rat CDH4. It is sometimes possible for the material contained within the vial of "Cadherin-4/R-Cadherin, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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