Mouse SEMA6C ELISA Kit | SEMA6C elisa kit

Mouse SEMA6C PicoKine ELISA Kit

Cat. Num. AAA1752663

• Gene Names: Sema6c; Semay; mKIAA1869
• Reactivity: Mouse
• Synonyms: SEMA6C; Mouse SEMA6C PicoKine ELISA Kit; Mouse SEMA6C; sema domain; transmembrane domain (TM); and cytoplasmic domain; (semaphorin) 6C; Semaphorin-6C; Semaphorin-Y; Sema Y; SEMA6C elisa kit

• Reactivity: Mouse
• Specificity: Natural and recombinant Mouse Sema6c
• Sequence Length: 963

• Samples: Cell culture supernates, serum and plasma (heparin, EDTA, citrate)
• Detection Range: 62.5pg/ml-4000pg/ml
• Sensitivity: <50pg/ml
• Intra-assay Precision: Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested on one plate to assess intra-assay precision.
• Inter-assay Precision: Inter-Assay Precision (Precision across assays):Three samples of known concentration were tested in separate assays to assess inter-assay precision.
• Preparation and Storage: Store at 4 degree C for 6 months. Store at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for SEMA6C elisa kit

Principle of the Assay: The Picokine™ Mouse Sema6c Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Mouse Sema6c with a 96-well strip plate that is pre-coated with antibody specific for Sema6c. The detection antibody is a biotinylated antibody specific for Sema6c. The capture antibody is a monoclonal antibody from rat, the detection antibody is a biotinylated polyclonal antibody from goat. The kit contains recombinant Mouse Sema6c with immunogen: Expression system for standard: NSO, Immunogen sequence: A26-P605. The kit is analytically validated with ready to use reagents. To measure Mouse Sema6c, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is substrate to HRP and will be catalyzed to produce a blue color product, which changes into yellow after adding acidic stop solution. The density of the yellow product is linearly proportional to Mouse Sema6c in the sample. Read the density of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Mouse Sema6c in the sample.!!Background/Introduction: Semaphorin-6C is a protein that in humans is encoded by the SEMA6C gene. This gene is mapped to 3; 3 F2.1. This gene product is a member of the semaphoring family of proteins. Semaphorins represent important molecular signals controlling multiple aspects of the cellular response that follows CNS injury, and thus may play an important role in neural regeneration.

NCBI and Uniprot Product Information

• NCBI GI #: 433660824
• NCBI GeneID: 20360
• NCBI Accession #: NP_001258953.1
• NCBI GenBank Nucleotide #: NP_001258953.1
• UniProt Accession #: Q9WTM3
• NCBI Official Full Name: semaphorin-6C isoform 1
• NCBI Official Synonym Full Names: sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6C
• NCBI Official Symbol: Sema6c
• NCBI Official Synonym Symbols: Semay; mKIAA1869
• NCBI Protein Information: semaphorin-6C
• UniProt Protein Name: Semaphorin-6C
• Protein Family: Semaphorin
• UniProt Gene Name: Sema6c
• UniProt Synonym Gene Names: Semay; Sema Y
• UniProt Entry Name: SEM6C_MOUSE

Uniprot Description

Function: May be a stop signal for the dorsal root ganglion neurons in their target areas, and possibly also for other neurons. May also be involved in the maintenance and remodeling of neuronal connections

By similarity.

Subcellular location: Cell membrane; Single-pass type I membrane protein.

Sequence similarities: Belongs to the semaphorin family.Contains 1 PSI domain.Contains 1 Sema domain.