Mouse Ceruloplasmin (CP) ELISA Kit | CP elisa kit

Mouse Ceruloplasmin (CP) ELISA Kit

Cat. Num. AAA453351

• Gene Names: Cp; D3Ertd555e
• Reactivity: Mouse
• Synonyms: Ceruloplasmin (CP); Mouse Ceruloplasmin (CP) ELISA Kit; CP2; CER; Caeruloplasmin; Ferroxidase; Oxygen Oxidoreductase; CP elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of CP. No significant cross-reactivity or interference between CP and analogues was observed.
• Sequence Length: 1085

• Samples: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
• Assay Type: Sandwich
• Detection Range: 0.781-50ng/mL
• Sensitivity: 0.27ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level CP were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level CP were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CP elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of CP in mouse serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to CP. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to CP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain CP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of CP in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 442745912
• NCBI GeneID: 12870
• NCBI Accession #: NP_001263177.1
• NCBI GenBank Nucleotide #: NM_001276248.1
• UniProt Accession #: Q61147; Q6P5C8
• Molecular Weight: 121,151 Da
• NCBI Official Full Name: ceruloplasmin isoform c
• NCBI Official Synonym Full Names: ceruloplasmin
• NCBI Official Symbol: Cp
• NCBI Official Synonym Symbols: D3Ertd555e
• NCBI Protein Information: ceruloplasmin
• UniProt Protein Name: Ceruloplasmin
• Protein Family: Ceruloplasmin
• UniProt Gene Name: Cp

NCBI Description

The protein encoded by this gene is a copper-containing glycoprotein found soluble in the serum and GPI-anchored in other tissues. It oxidizes Fe(II) to Fe(III) and is proposed to play an important role in iron homeostasis. In humans mutations of this gene cause aceruloplasminemia, which is characterized by retinal degeneration, diabetes, anemia and neurological symptoms. In mouse deficiency of this gene in combination with a deficiency of its homolog hephaestin causes retinal degeneration and serves as a pathophysiological model for aceruloplasminemia and age-related macular degeneration. Alternative splicing results in multiple transcript variants that encode different protein isoforms. [provided by RefSeq, Jan 2013]

Uniprot Description

Ceruloplasmin is a blue, copper-binding (6-7 atoms per molecule) glycoprotein. It has ferroxidase activity oxidizing Fe2+ to Fe3+ without releasing radical oxygen species. It is involved in iron transport across the cell membrane. Provides Cu2+ ions for the ascorbate-mediated deaminase degradation of the heparan sulfate chains of GPC1. May also play a role in fetal lung development or pulmonary antioxidant defense ().