Rat Peroxiredoxin 5 (PRDX5) ELISA Kit | PRDX5 elisa kit

Rat Peroxiredoxin 5 (PRDX5) ELISA Kit

Cat. Num. AAA2098083

• Gene Names: Prdx5; Aoeb166
• Reactivity: Rat
• Synonyms: Peroxiredoxin 5 (PRDX5); Rat Peroxiredoxin 5 (PRDX5) ELISA Kit; PLP; PRDX6; ACR1; PMP20; PRXV; SBBI10; Thioredoxin Peroxidase PMP20; Peroxisomal Antioxidant Enzyme; TPx Type VI; Liver Tissue 2D-Page Spot 71B; Alu Co-Repressor 1; PRDX5 elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of Peroxiredoxin 5 (PRDX5).; No significant cross-reactivity or interference between Peroxiredoxin 5 (PRDX5) and analogues was observed.
• Sequence Length: 213

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma, Tissue homogenates, Cell lysates, Cell culture supernates and Other Biological Fluids
• Detection Range: 78-5,000pg/mL
• Sensitivity: < 34pg/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Peroxiredoxin 5 (PRDX5) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Peroxiredoxin 5 (PRDX5) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for PRDX5 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Peroxiredoxin 5 (PRDX5). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Peroxiredoxin 5 (PRDX5). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Peroxiredoxin 5 (PRDX5), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Peroxiredoxin 5 (PRDX5) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for PRDX5 elisa kit

Enzyme & Kinase

NCBI and Uniprot Product Information

• NCBI GI #: 16758404
• NCBI GeneID: 113898
• NCBI Accession #: NP_446062.1
• NCBI GenBank Nucleotide #: NM_053610.1
• UniProt Accession #: Q9R063; Q68G22
• Molecular Weight: 17,035 Da
• NCBI Official Full Name: peroxiredoxin-5, mitochondrial
• NCBI Official Synonym Full Names: peroxiredoxin 5
• NCBI Official Symbol: Prdx5
• NCBI Official Synonym Symbols: Aoeb166
• NCBI Protein Information: peroxiredoxin-5, mitochondrial
• UniProt Protein Name: Peroxiredoxin-5, mitochondrial
• Protein Family: Peroxiredoxin
• UniProt Gene Name: Prdx5
• UniProt Synonym Gene Names: AOEB166; Prx-V

NCBI Description

member of the AhpC/TSA peroxiredoxin family with mitochondrial and peroxisomal sorting signals; may play an antioxidant protective role in various tissues [RGD, Feb 2006]

Uniprot Description

PRDX5: Reduces hydrogen peroxide and alkyl hydroperoxides with reducing equivalents provided through the thioredoxin system. Involved in intracellular redox signaling. Belongs to the peroxiredoxin 2 family. 2 isoforms of the human protein are produced by alternative initiation.

Protein type: EC 1.11.1.15; Oxidoreductase

Chromosomal Location of Human Ortholog: 1q43

Cellular Component: cytoplasm; cytoplasmic vesicle; cytosol; extracellular space; intracellular membrane-bound organelle; mitochondrion; nucleus; perinuclear region of cytoplasm; peroxisomal matrix; peroxisome

Molecular Function: antioxidant activity; caspase inhibitor activity; peroxidase activity; peroxiredoxin activity; protein dimerization activity; receptor binding; thioredoxin peroxidase activity

Biological Process: apoptosis; cell redox homeostasis; hydrogen peroxide catabolic process; negative regulation of apoptosis; negative regulation of cysteine-type endopeptidase activity involved in apoptotic process; negative regulation of oxidoreductase activity; negative regulation of transcription from RNA polymerase III promoter; positive regulation of collagen biosynthetic process; response to oxidative stress