Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Peroxiredoxin-5, mitochondrial (PRDX5) Standard Curve.)
Mouse Peroxiredoxin-5, Mitochondrial (PRDX5) ELISA Kit | PRDX5 elisa kit
Mouse Peroxiredoxin-5, Mitochondrial (PRDX5) ELISA Kit
Gene Names
Prdx5; PLP; AOPP; PrxV; Pmp20; Prdx6; AOEB166
Reactivity
Mouse
Synonyms
Peroxiredoxin-5; Mitochondrial (PRDX5); Mouse Peroxiredoxin-5; Mitochondrial (PRDX5) ELISA Kit; PRDX5; ACR1; AOEB166; B166; MGC117264; MGC142283; MGC142285; PLP; PMP20; PRDX6; PRXV; SBBI10; Alu co-repressor 1; TPx type VI; antioxidant enzyme B166; liver tissue 2D-page spot 71B; peroxisomal antioxidant en; PRDX5 elisa kit
Reactivity
Mouse
Specificity
Mouse Peroxiredoxin-5, mitochondrial (PRDX5) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse PRDX5. No significant cross-reactivity or interference between Mouse PRDX5 and analogues was observed.
Sequence Length
210
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Peroxiredoxin-5, mitochondrial (PRDX5) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Peroxiredoxin-5, mitochondrial (PRDX5) Standard Curve.)
Related Product Information for PRDX5 elisa kit
Description: This Mouse Peroxiredoxin-5, mitochondrial (PRDX5) ELISA Kit employs a two-site sandwich ELISA to quantitate PRDX5 in samples. An antibody specific for PRDX5 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PRDX5 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PRDX5 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PRDX5 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Peroxiredoxin-5, mitochondrial is a member of the peroxiredoxin family of antioxidant enzymes, which reduce hydrogen peroxide and alkyl hydroperoxides. The encoded protein may play an antioxidant protective role in different tissues under normal conditions and during inflammatory processes. This protein interacts with peroxisome receptor 1. The crystal structure of this protein in its reduced form has been resolved to 1.5 angstrom resolution. This gene uses alternate in-frame translation initiation sites to generate mitochondrial or peroxisomal/cytoplasmic forms. Three transcript variants encoding distinct isoforms have been identified for this gene.
Background: Peroxiredoxin-5, mitochondrial is a member of the peroxiredoxin family of antioxidant enzymes, which reduce hydrogen peroxide and alkyl hydroperoxides. The encoded protein may play an antioxidant protective role in different tissues under normal conditions and during inflammatory processes. This protein interacts with peroxisome receptor 1. The crystal structure of this protein in its reduced form has been resolved to 1.5 angstrom resolution. This gene uses alternate in-frame translation initiation sites to generate mitochondrial or peroxisomal/cytoplasmic forms. Three transcript variants encoding distinct isoforms have been identified for this gene.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
peroxiredoxin-5, mitochondrial isoform 1
NCBI Official Synonym Full Names
peroxiredoxin 5
NCBI Official Symbol
Prdx5
NCBI Official Synonym Symbols
PLP; AOPP; PrxV; Pmp20; Prdx6; AOEB166
NCBI Protein Information
peroxiredoxin-5, mitochondrial
UniProt Protein Name
Peroxiredoxin-5, mitochondrial
Protein Family
UniProt Gene Name
Prdx5
UniProt Synonym Gene Names
Prdx6; AOEB166; Prx-V
UniProt Entry Name
PRDX5_MOUSE
NCBI Description
This gene encodes a member of the peroxiredoxin family of antioxidant enzymes, which reduce hydrogen peroxide and alkyl hydroperoxides. The encoded protein plays an antioxidant protective role in different tissues under normal conditions and during inflammatory processes. The use of alternate transcription start sites may result in use of alternate in-frame translation start codons that generate alternate isoforms that are targeted to the mitochondrion or peroxisome/cytoplasm. [provided by RefSeq, Nov 2017]
Research Articles on PRDX5
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Product Notes
The Mouse PRDX5 prdx5 (Catalog #AAA9717301) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9717301 ELISA Kit recognizes Mouse PRDX5. It is sometimes possible for the material contained within the vial of "Peroxiredoxin-5, Mitochondrial (PRDX5), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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