Typical Testing Data/Standard Curve (for reference only)
Human PRDX5 ELISA Kit | PRDX5 elisa kit
Human PRDX5 (Peroxiredoxin 5) ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human PRDX5. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human PRDX5 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human PRDX5, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human PRDX5. You can calculate the concentration of Human PRDX5 in the samples by comparing the OD of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the peroxiredoxin family of antioxidant enzymes, which reduce hydrogen peroxide and alkyl hydroperoxides. The encoded protein may play an antioxidant protective role in different tissues under normal conditions and during inflammatory processes. This protein interacts with peroxisome receptor 1. The crystal structure of this protein in its reduced form has been resolved to 1.5 angstrom resolution. This gene uses alternate in-frame translation initiation sites to generate mitochondrial or peroxisomal/cytoplasmic forms. Three transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Jul 2008]
Uniprot Description
PRDX5: Reduces hydrogen peroxide and alkyl hydroperoxides with reducing equivalents provided through the thioredoxin system. Involved in intracellular redox signaling. Belongs to the peroxiredoxin 2 family. 2 isoforms of the human protein are produced by alternative initiation.
Protein type: EC 1.11.1.15; Oxidoreductase
Chromosomal Location of Human Ortholog: 11q13
Cellular Component: extracellular space; peroxisomal matrix; intracellular membrane-bound organelle; mitochondrion; perinuclear region of cytoplasm; mitochondrial matrix; cytoplasm; peroxisome; cytoplasmic vesicle; nucleus; cytosol
Molecular Function: protein dimerization activity; antioxidant activity; peroxidase activity; peroxiredoxin activity; caspase inhibitor activity; receptor binding
Biological Process: response to reactive oxygen species; hydrogen peroxide catabolic process; negative regulation of oxidoreductase activity; negative regulation of transcription from RNA polymerase III promoter; positive regulation of collagen biosynthetic process; negative regulation of caspase activity; response to oxidative stress; inflammatory response; negative regulation of apoptosis
Research Articles on PRDX5
Similar Products
Product Notes
The Human PRDX5 prdx5 (Catalog #AAA2513779) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2513779 ELISA Kit recognizes Human PRDX5. It is sometimes possible for the material contained within the vial of "PRDX5, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
