Rabbit IRAK4 Polyclonal Antibody | anti-IRAK4 antibody

Rabbit anti IRAK4

Cat. Num. AAA225124

• Gene Names: IRAK4; IPD1; REN64; IRAK-4; NY-REN-64
• Reactivity: Human, Mouse (Expected from Sequence), Rat (Expected from Sequence)
• Applications: Western Blot
• Purity: Purified whole serum by affinity chromatography.
• Synonyms: IRAK4; Polyclonal Antibody; Rabbit anti IRAK4; IRAK4 Antibody; anti-IRAK4 antibody

• Host: Rabbit
• Reactivity: Human, Mouse (Expected from Sequence), Rat (Expected from Sequence)
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified whole serum by affinity chromatography.
• Form/Format: Liquid in phosphate buffered saline with 0.02% sodium azide, 50% glycerol.
• Sequence Length: 460

• Applicable Applications for anti-IRAK4 antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:2000
• Immunogen: Recombinant human IRAK4
• Preperation: Antiserum to IRAK4 was raised by repeated immunization of rabbits with highly purified antigen.
• Preparation and Storage: Store at -20 degree C.; Storage in frost-free freezers is not recommended. This product should be stored undiluted.; Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. Shelf Life: 12 months from date of despatch

Western Blot (WB)

(Western blot analysis of cell lysates using Rabbit anti IRAK4 antibody)

Related Product Information for anti-IRAK4 antibody

Rabbit anti IRAK4 antibody recognizes human IRAK4, also known as interleukin-1 receptor associated kinase-like 4. IRAK4 unlike IRAK1-3 lacks the C-terminal domain required for TRAF6 interaction.

NCBI and Uniprot Product Information

• NCBI GI #: 50401181
• NCBI GeneID: 51135
• UniProt Accession #: Q9NWZ3; Q69FE1; Q8TDF7; Q9Y589
• Molecular Weight: 37,674 Da
• NCBI Official Full Name: Interleukin-1 receptor-associated kinase 4
• NCBI Official Synonym Full Names: interleukin 1 receptor associated kinase 4
• NCBI Official Symbol: IRAK4
• NCBI Official Synonym Symbols: IPD1; REN64; IRAK-4; NY-REN-64
• NCBI Protein Information: interleukin-1 receptor-associated kinase 4
• UniProt Protein Name: Interleukin-1 receptor-associated kinase 4
• Protein Family: Interleukin-1 receptor-associated kinase
• UniProt Gene Name: IRAK4
• UniProt Synonym Gene Names: IRAK-4

NCBI Description

This gene encodes a kinase that activates NF-kappaB in both the Toll-like receptor (TLR) and T-cell receptor (TCR) signaling pathways. The protein is essential for most innate immune responses. Mutations in this gene result in IRAK4 deficiency and recurrent invasive pneumococcal disease. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2011]

Uniprot Description

Serine/threonine-protein kinase that plays a critical role in initiating innate immune response against foreign pathogens. Involved in Toll-like receptor (TLR) and IL-1R signaling pathways (PubMed:17878374). Is rapidly recruited by MYD88 to the receptor-signaling complex upon TLR activation to form the Myddosome together with IRAK2. Phosphorylates initially IRAK1, thus stimulating the kinase activity and intensive autophosphorylation of IRAK1. Phosphorylates E3 ubiquitin ligases Pellino proteins (PELI1, PELI2 and PELI3) to promote pellino-mediated polyubiquitination of IRAK1. Then, the ubiquitin-binding domain of IKBKG/NEMO binds to polyubiquitinated IRAK1 bringing together the IRAK1-MAP3K7/TAK1-TRAF6 complex and the NEMO-IKKA-IKKB complex. In turn, MAP3K7/TAK1 activates IKKs (CHUK/IKKA and IKBKB/IKKB) leading to NF-kappa-B nuclear translocation and activation. Alternatively, phosphorylates TIRAP to promote its ubiquitination and subsequent degradation. Phosphorylates NCF1 and regulates NADPH oxidase activation after LPS stimulation suggesting a similar mechanism during microbial infections.