Rat Neuritin 1 (NRN1) ELISA Kit | NRN1 elisa kit

Rat Neuritin 1 (NRN1) ELISA Kit

Cat. Num. AAA2019251

• Gene Names: NRN1; NRN; dJ380B8.2
• Reactivity: Rat
• Synonyms: Neuritin 1 (NRN1); Rat Neuritin 1 (NRN1) ELISA Kit; NRN1 elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of Neuritin 1 (NRN1).; No significant cross-reactivity or interference between Neuritin 1 (NRN1) and analogues was observed.
• Sequence Length: 142

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma, Tissue homogenates, Cell lysates and Other Biological Fluids
• Detection Range: 31.25-2000pg/mL
• Sensitivity: < 13.5pg/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Neuritin 1 (NRN1) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Neuritin 1 (NRN1) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for NRN1 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Neuritin 1 (NRN1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Neuritin 1 (NRN1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Neuritin 1 (NRN1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Neuritin 1 (NRN1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for NRN1 elisa kit

Neuroscience

NCBI and Uniprot Product Information

• NCBI GI #: 525314165
• NCBI GeneID: 51299
• NCBI Accession #: NP_001265639.1
• NCBI GenBank Nucleotide #: NM_001278710.1
• Molecular Weight: 15,333 Da
• NCBI Official Full Name: neuritin isoform 1
• NCBI Official Synonym Full Names: neuritin 1
• NCBI Official Symbol: NRN1
• NCBI Official Synonym Symbols: NRN; dJ380B8.2
• NCBI Protein Information: neuritin
• UniProt Protein Name: Neuritin
• Protein Family: Neuritin
• UniProt Gene Name: NRN1
• UniProt Synonym Gene Names: NRN
• UniProt Entry Name: NRN1_HUMAN

NCBI Description

This gene encodes a member of the neuritin family, and is expressed in postmitotic-differentiating neurons of the developmental nervous system and neuronal structures associated with plasticity in the adult. The expression of this gene can be induced by neural activity and neurotrophins. The encoded protein contains a consensus cleavage signal found in glycosylphoshatidylinositol (GPI)-anchored proteins. The encoded protein promotes neurite outgrowth and arborization, suggesting its role in promoting neuritogenesis. Overexpression of the encoded protein may be associated with astrocytoma progression. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]

Uniprot Description

NRN1: Promotes neurite outgrowth and especially branching of neuritic processes in primary hippocampal and cortical cells. Belongs to the neuritin family.

Protein type: Membrane protein, GPI anchor

Chromosomal Location of Human Ortholog: 6p25.1

Cellular Component: plasma membrane; synapse; cell junction

Biological Process: nervous system development