Human MMP-8 ELISA Kit | MMP8 elisa kit

Human MMP-8 Quick ELISA Kit

Cat. Num. AAA1753918

• Gene Names: MMP8; HNC; CLG1; MMP-8; PMNL-CL
• Reactivity: Human
• Synonyms: MMP-8; Human MMP-8 Quick ELISA Kit; MMP-8|CLG1HNC; Collagenase 2; EC 3. 4. 24; EC 3. 4. 24. 34; matrix metallopeptidase 8 (neutrophil collagenase); matrix metalloproteinase 8 (neutrophil collagenase); Matrix metalloproteinase-8; MMP8; neutrophil collagenase; PMNL collagenase; PMNL-CL; MMP8 elisa kit

• Reactivity: Human
• Specificity: Natural and recombinant Human MMP8

• Samples: Cell culture supernatants, serum, plasma (heparin) and saliva
• Assay Type: Sandwich
• Detection Range: 156 pg/ml - 10,000 pg/ml
• Sensitivity: <10 pg/ml
• Intra-assay Precision: Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested on one plate to assess intra-assay precision.
• Inter-assay Precision: Inter-Assay Precision (Precision across assays): Three samples of known concentration were tested in separate assays to assess inter-assay precision.
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles. (Shipped with wet ice.)

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for MMP8 elisa kit

Principle of the Assay: The Quick Picokine™ Human MMP8 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Human MMP8 in cell culture supernatants, serum, plasma (heparin) and saliva. It uses our proprietary Quick ELISA technology. Quick ELISA technology employs capture antibodies conjugated to an affinity tag that is recognized by the polyclonal antibody used to coat our Quick ELISA plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. The kit contains recombinant Human MMP8 with immunogen: Expression system for standard: NS0; Immunogen sequence: F21-G467. To measure Human MMP8, add standards and samples to the wells, then add antibody cocktail. Wash the wells with PBS or TBS buffer, and add TMB. TMB is an HRP substrate and will be catalyzed to produce a blue color product, which changes into yellow after adding the acidic stop solution. The absorbance of the yellow product is linearly proportional to Human MMP8 in the sample. Read the absorbance of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Human MMP8 in the sample.

Background/Introduction: Matrix metalloproteinase 8 (MMP8) also called neutrophil collagenase. Neutrophil collagenase, a member of the family of matrix metalloproteinases, is distinct from the collagenase of skin fibroblasts and synovial cells in substrate specificity and immunologic crossreactivity. MMP8, an enzyme that degrades fibrillar collagens imparting strength to the fetal membranes, is expressed by leukocytes and chorionic cytotrophoblast cells. The human neutrophil collagenase (HNC) cDNA clone has been sequenced and shown to encode a 467-residue protein. Neutrophil collagenase has been found to possess 57% identity with the deduced protein sequence for fibroblast collagenase with 72% chemical similarity. Certain regions of the molecule, including the putative zinc-binding region, are highly conserved. When compared with the published sequence for fibroblast collagenase, neutrophil collagenase contains four additional sites for glycosylation. The standard product used in this kit is natural, isolating from human MMP-8. The detected MMP-8 includes zymogen and active enzyme.

Product Categories/Family for MMP8 elisa kit

ELISA kits

NCBI and Uniprot Product Information

• NCBI GI #: 4505221
• NCBI GeneID: 4317
• NCBI Accession #: NP_002415.1
• NCBI GenBank Nucleotide #: NM_002424.2
• UniProt Accession #: P22894; Q45F99
• Molecular Weight: 53,412 Da
• NCBI Official Full Name: neutrophil collagenase preproprotein
• NCBI Official Synonym Full Names: matrix metallopeptidase 8 (neutrophil collagenase)
• NCBI Official Symbol: MMP8
• NCBI Official Synonym Symbols: HNC; CLG1; MMP-8; PMNL-CL
• NCBI Protein Information: neutrophil collagenase; PMNL collagenase; matrix metalloproteinase-8; matrix metalloproteinase 8 (neutrophil collagenase)
• UniProt Protein Name: Neutrophil collagenase
• Protein Family: Neutrophil collagenase
• UniProt Gene Name: MMP8
• UniProt Synonym Gene Names: CLG1; MMP-8; PMNL-CL
• UniProt Entry Name: MMP8_HUMAN

NCBI Description

Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. However, the enzyme encoded by this gene is stored in secondary granules within neutrophils and is activated by autolytic cleavage. Its function is degradation of type I, II and III collagens. The gene is part of a cluster of MMP genes which localize to chromosome 11q22.3. [provided by RefSeq, Jul 2008]

Uniprot Description

MMP8: Can degrade fibrillar type I, II, and III collagens. Belongs to the peptidase M10A family.

Protein type: Secreted; EC 3.4.24.34; Protease; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 11q22.3

Cellular Component: proteinaceous extracellular matrix; extracellular space; extracellular region

Molecular Function: zinc ion binding; metalloendopeptidase activity; serine-type endopeptidase activity; calcium ion binding

Biological Process: collagen catabolic process; extracellular matrix disassembly; extracellular matrix organization and biogenesis; proteolysis