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Immunohistochemistry (IHC) (Human Prostate )

Rabbit NRF1 Polyclonal Antibody | anti-NRF1 antibody

NRF1 Antibody - middle region

Gene Names
NRF1; ALPHA-PAL
Reactivity
Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat, Zebrafish
Applications
Western Blot, Immunohistochemistry
Purity
Affinity Purified
Synonyms
NRF1; Polyclonal Antibody; NRF1 Antibody - middle region; anti-NRF1 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat, Zebrafish
Clonality
Polyclonal
Purity/Purification
Affinity Purified
Form/Format
Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Sequence
Synthetic peptide located within the following region: WATLQGGEMTIQTTQASEATQAVASLAEAAVAASQEMQQGATVTMALNSE
Sequence Length
503
Applicable Applications for anti-NRF1 antibody
Western Blot (WB), Immunohistochemistry (IHC)
Homology
Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%; Zebrafish: 100%
Immunogen
The immunogen is a synthetic peptide directed towards the middle region of Human NRF1
Preparation and Storage
For short term use, store at 2-8 degree C up to 1 week. For long term storage, store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Immunohistochemistry (IHC)

(Human Prostate )

Immunohistochemistry (IHC) (Human Prostate )

Immunohistochemistry (IHC)

(Rabbit Anti-NRF1 AntibodyFormalin Fixed Paraffin Embedded Tissue: Human Adult heartObserved Staining: NuclearPrimary Antibody Concentration: 1:600Secondary Antibody: Donkey anti-Rabbit-Cy2/3Secondary Antibody Concentration: 1:200Magnification: 20XExposure Time: 0.5- 2.0 secProtocol located in Reviews and Data.)

Immunohistochemistry (IHC) (Rabbit Anti-NRF1 AntibodyFormalin Fixed Paraffin Embedded Tissue: Human Adult heartObserved Staining: NuclearPrimary Antibody Concentration: 1:600Secondary Antibody: Donkey anti-Rabbit-Cy2/3Secondary Antibody Concentration: 1:200Magnification: 20XExposure Time: 0.5- 2.0 secProtocol located in Reviews and Data.)

Immunohistochemistry (IHC)

(Rabbit Anti-NRF1 AntibodyFormalin Fixed Paraffin Embedded Tissue: Human Adult liverObserved Staining: Nuclear and some weak cytoplasmicPrimary Antibody Concentration: 1:600Secondary Antibody: Donkey anti-Rabbit-Cy2/3Secondary Antibody Concentration: 1:200Magnification: 20XExposure Time: 0.5 -2.0 secProtocol located in Reviews and Data.)

Immunohistochemistry (IHC) (Rabbit Anti-NRF1 AntibodyFormalin Fixed Paraffin Embedded Tissue: Human Adult liverObserved Staining: Nuclear and some weak cytoplasmicPrimary Antibody Concentration: 1:600Secondary Antibody: Donkey anti-Rabbit-Cy2/3Secondary Antibody Concentration: 1:200Magnification: 20XExposure Time: 0.5 -2.0 secProtocol located in Reviews and Data.)

Western Blot (WB)

(Host: MouseTarget Name: NRF1Sample Tissue: Mouse HeartAntibody Dilution: 1ug/ml)

Western Blot (WB) (Host: MouseTarget Name: NRF1Sample Tissue: Mouse HeartAntibody Dilution: 1ug/ml)

Western Blot (WB)

(Human 293TThere is BioGPS gene expression data showing that NRF1 is expressed in HEK293T)

Western Blot (WB) (Human 293TThere is BioGPS gene expression data showing that NRF1 is expressed in HEK293T)

Western Blot (WB)

(Human HelaThere is BioGPS gene expression data showing that NRF1 is expressed in HeLa)

Western Blot (WB) (Human HelaThere is BioGPS gene expression data showing that NRF1 is expressed in HeLa)

Western Blot (WB)

(Human JurkatThere is BioGPS gene expression data showing that NRF1 is expressed in Jurkat)

Western Blot (WB) (Human JurkatThere is BioGPS gene expression data showing that NRF1 is expressed in Jurkat)

Western Blot (WB)

(WB Suggested Anti-NRF1 Antibody Titration: 0.2-1 ug/mlELISA Titer: 1:62500Positive Control: Transfected 293T)

Western Blot (WB) (WB Suggested Anti-NRF1 Antibody Titration: 0.2-1 ug/mlELISA Titer: 1:62500Positive Control: Transfected 293T)

Western Blot (WB)

(WB Suggested Anti-NRF1 antibody Titration: 1 ug/mLSample Type: Human heart)

Western Blot (WB) (WB Suggested Anti-NRF1 antibody Titration: 1 ug/mLSample Type: Human heart)
Related Product Information for anti-NRF1 antibody
This is a rabbit polyclonal antibody against NRF1. It was validated on Western Blot

Target Description: This protein homodimerizes and functions as a transcription factor which activates the expression of some key metabolic genes regulating cellular growth and nuclear genes required for respiration, heme biosynthesis, and mitochondrial DNA transcription and replication. The protein has also been associated with the regulation of neurite outgrowth.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
53kDa
NCBI Official Full Name
nuclear respiratory factor 1 isoform 1
NCBI Official Synonym Full Names
nuclear respiratory factor 1
NCBI Official Symbol
NRF1
NCBI Official Synonym Symbols
ALPHA-PAL
NCBI Protein Information
nuclear respiratory factor 1
UniProt Protein Name
Nuclear respiratory factor 1
UniProt Gene Name
NRF1
UniProt Synonym Gene Names
NRF-1; Alpha-pal
UniProt Entry Name
NRF1_HUMAN

NCBI Description

This gene encodes a protein that homodimerizes and functions as a transcription factor which activates the expression of some key metabolic genes regulating cellular growth and nuclear genes required for respiration, heme biosynthesis, and mitochondrial DNA transcription and replication. The protein has also been associated with the regulation of neurite outgrowth. Alternative splicing results in multiple transcript variants. Confusion has occurred in bibliographic databases due to the shared symbol of NRF1 for this gene and for "nuclear factor (erythroid-derived 2)-like 1" which has an official symbol of NFE2L1. [provided by RefSeq, May 2014]

Uniprot Description

NRF1: Transcription factor that activates the expression of the EIF2S1 (EIF2-alpha) gene. Links the transcriptional modulation of key metabolic genes to cellular growth and development. Implicated in the control of nuclear genes required for respiration, heme biosynthesis, and mitochondrial DNA transcription and replication. Belongs to the NRF1/Ewg family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 7q32

Cellular Component: nucleoplasm; cytoplasm

Biological Process: regulation of transcription from RNA polymerase II promoter; mitochondrion organization and biogenesis; organ regeneration; generation of precursor metabolites and energy; transcription, DNA-dependent; response to folic acid; organelle organization and biogenesis; response to hypoxia; positive regulation of transcription from RNA polymerase II promoter; response to lipopolysaccharide; cellular lipid metabolic process; response to electrical stimulus; response to estradiol stimulus

Research Articles on NRF1

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Product Notes

The NRF1 nrf1 (Catalog #AAA3202113) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The NRF1 Antibody - middle region reacts with Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat, Zebrafish and may cross-react with other species as described in the data sheet. AAA Biotech's NRF1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). Researchers should empirically determine the suitability of the NRF1 nrf1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Synthetic peptide located within the following region: WATLQGGEMT IQTTQASEAT QAVASLAEAA VAASQEMQQG ATVTMALNSE. It is sometimes possible for the material contained within the vial of "NRF1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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