Keratin 18 (KRT18) ELISA Kit | KRT18 elisa kit

Rat Keratin 18 (KRT18) ELISA Kit

Cat. Num. AAA2022671

• Gene Names: KRT18; K18; CK-18; CYK18
• Reactivity: Rat
• Synonyms: Keratin 18 (KRT18); Rat Keratin 18 (KRT18) ELISA Kit; CK18; CYK18; K18; Cytokeratin 18; Cell proliferation-inducing gene 46 protein; Keratin; type I cytoskeletal 18; KRT18 elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of Keratin 18 (KRT18).; No significant cross-reactivity or interference between Keratin 18 (KRT18) and analogues was observed.
• Sequence Length: 430

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma, Tissue homogenates, Cell lysates, Cell culture supernates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.056ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Keratin 18 (KRT18) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Keratin 18 (KRT18) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for KRT18 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Keratin 18 (KRT18). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Keratin 18 (KRT18). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Keratin 18 (KRT18), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Keratin 18 (KRT18) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for KRT18 elisa kit

Tumor immunity; Pulmonology; Dermatology

NCBI and Uniprot Product Information

• NCBI GI #: 40354195
• NCBI GeneID: 3875
• NCBI Accession #: NP_954657.1
• NCBI GenBank Nucleotide #: NM_199187.1
• UniProt Accession #: P05783; Q53G38; Q5U0N8; Q9BW26
• Molecular Weight: 48,058 Da
• NCBI Official Full Name: keratin, type I cytoskeletal 18
• NCBI Official Synonym Full Names: keratin 18, type I
• NCBI Official Symbol: KRT18
• NCBI Official Synonym Symbols: K18; CK-18; CYK18
• NCBI Protein Information: keratin, type I cytoskeletal 18
• UniProt Protein Name: Keratin, type I cytoskeletal 18
• Protein Family: Keratin
• UniProt Gene Name: KRT18
• UniProt Synonym Gene Names: CYK18; CK-18; K18
• UniProt Entry Name: K1C18_HUMAN

NCBI Description

KRT18 encodes the type I intermediate filament chain keratin 18. Keratin 18, together with its filament partner keratin 8, are perhaps the most commonly found members of the intermediate filament gene family. They are expressed in single layer epithelial tissues of the body. Mutations in this gene have been linked to cryptogenic cirrhosis. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

K18: a type I cytoskeletal keratin. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. Keratin 18 and its filament partner keratin 8 are perhaps the most commonly found members of the intermediate filament gene family. They are expressed in single layer epithelial tissues of the body. Defects are a cause of cryptogenic cirrhosis.

Protein type: Cytoskeletal; Nucleolus

Chromosomal Location of Human Ortholog: 12q13

Cellular Component: perinuclear region of cytoplasm; keratin filament; cytoplasm; nucleolus; microtubule organizing center; intermediate filament

Molecular Function: protein binding; structural molecule activity

Biological Process: anatomical structure morphogenesis; viral reproduction; tumor necrosis factor-mediated signaling pathway; intermediate filament cytoskeleton organization and biogenesis; cell cycle; Golgi to plasma membrane CFTR protein transport; negative regulation of apoptosis

Disease: Cirrhosis, Familial