Western Blot (WB)
(Western blot analysis of extracts of Human coronavirus HKU1 (isolate N5) (HCoV-HKU1) Spike Protein (S1+S2 ECDHis Tag), using HCoV-HKU1 Spike S1 antibody at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 180s.)
Rabbit HCoV-HKU1 Spike S1 Polyclonal Antibody | anti-HCoV-HKU1 antibody
HCoV-HKU1 Spike S1 Rabbit pAb
Reactivity
HCoV-HKU1
Applications
Western Blot
Purity
Affinity purification
Synonyms
HCoV-HKU1 Spike S1; Polyclonal Antibody; HCoV-HKU1 Spike S1 Rabbit pAb; anti-HCoV-HKU1 antibody
Host
Rabbit
Reactivity
HCoV-HKU1
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity purification
Form/Format
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Applicable Applications for anti-HCoV-HKU1 antibody
Western Blot (WB)
Application Notes
WB: 1:500-1:2000
Immunogen
Recombinant protein of HCoV-HKU1 Spike S1.
Positive Samples
Human coronavirus HKU1 (isolate N5)(HCoV-HKU1) Spike Protein (S1+S2 ECDHis Tag)
Preparation and Storage
Store at -20 degree C. Avoid freeze/thaw cycles.
Western Blot (WB)
(Western blot analysis of extracts of Human coronavirus HKU1 (isolate N5) (HCoV-HKU1) Spike Protein (S1+S2 ECDHis Tag), using HCoV-HKU1 Spike S1 antibody at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 180s.)
Western Blot (WB)
(Western blot analysis of extracts of Human coronavirus HKU1 (isolate N5) (HCoV-HKU1) Spike Protein (S1+S2 ECDHis Tag), using HCoV-HKU1 Spike S1 antibody at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 180s.)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
151,711 Da
NCBI Official Full Name
spike glycoprotein
NCBI Official Symbol
S
NCBI Protein Information
spike glycoprotein
UniProt Protein Name
Spike glycoprotein
UniProt Gene Name
S
UniProt Synonym Gene Names
S glycoprotein
Uniprot Description
S1 attaches the virion to the cell membrane by interacting with cell receptors, initiating the infection.
Research Articles on HCoV-HKU1
Similar Products
Product Notes
The HCoV-HKU1 s (Catalog #AAA9143755) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The HCoV-HKU1 Spike S1 Rabbit pAb reacts with HCoV-HKU1 and may cross-react with other species as described in the data sheet. AAA Biotech's HCoV-HKU1 Spike S1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:500-1:2000. Researchers should empirically determine the suitability of the HCoV-HKU1 s for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HCoV-HKU1 Spike S1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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