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Typical Testing Data

Mouse Keratin, type I cytoskeletal 18 ELISA Kit | Krt18 elisa kit

Mouse Keratin, type I cytoskeletal 18 ELISA Kit

Gene Names
KRT18; K18; CK-18; CYK18
Reactivity
Mouse
Synonyms
Keratin; type I cytoskeletal 18; Mouse Keratin; type I cytoskeletal 18 ELISA Kit; Krt18/CK-18 (Cytokeratin 18)/Krt18 (Kerd/Krt1-18)/Keratin; type I cytoskeletal 18/Keratin-18 (K18)/Cytokeratin endo B (Keratin D)/Cytokeratin-18 (CK-18)/; Krt18 elisa kit
Ordering
For Research Use Only!
Reactivity
Mouse
Sequence Length
430
Samples
Serum, plasma and other biological fluids.
Assay Type
Sandwich ELISA, Double Antibody
Detection Range
0.78-50ng/ml
Sensitivity
0.469ng/ml
Preparation and Storage
Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Testing Data

Typical Standard Curve

Typical Standard Curve
Related Product Information for Krt18 elisa kit
Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. anti-Mouse Krt18 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Mouse Krt18 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Mouse Krt18 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Mouse Krt18 can be calculated.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
48,058 Da
NCBI Official Full Name
keratin, type I cytoskeletal 18
NCBI Official Synonym Full Names
keratin 18, type I
NCBI Official Symbol
KRT18
NCBI Official Synonym Symbols
K18; CK-18; CYK18
NCBI Protein Information
keratin, type I cytoskeletal 18
UniProt Protein Name
Keratin, type I cytoskeletal 18
Protein Family
UniProt Gene Name
KRT18
UniProt Synonym Gene Names
CYK18; CK-18; K18
UniProt Entry Name
K1C18_HUMAN

NCBI Description

KRT18 encodes the type I intermediate filament chain keratin 18. Keratin 18, together with its filament partner keratin 8, are perhaps the most commonly found members of the intermediate filament gene family. They are expressed in single layer epithelial tissues of the body. Mutations in this gene have been linked to cryptogenic cirrhosis. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

K18: a type I cytoskeletal keratin. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. Keratin 18 and its filament partner keratin 8 are perhaps the most commonly found members of the intermediate filament gene family. They are expressed in single layer epithelial tissues of the body. Defects are a cause of cryptogenic cirrhosis.

Protein type: Nucleolus; Cytoskeletal

Chromosomal Location of Human Ortholog: 12q13

Cellular Component: cytoplasm; intermediate filament; keratin filament; microtubule organizing center; nucleolus; perinuclear region of cytoplasm

Molecular Function: protein binding; structural molecule activity

Biological Process: anatomical structure morphogenesis; cell cycle; Golgi to plasma membrane CFTR protein transport; intermediate filament cytoskeleton organization and biogenesis; negative regulation of apoptosis; tumor necrosis factor-mediated signaling pathway; viral reproduction

Disease: Cirrhosis, Familial

Research Articles on Krt18

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Product Notes

The Mouse Krt18 krt18 (Catalog #AAA761240) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA761240 ELISA Kit recognizes Mouse Krt18. It is sometimes possible for the material contained within the vial of "Keratin, type I cytoskeletal 18, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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