Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Kallikrein-6 (KLK6) Standard Curve.)
Rat Kallikrein-6 (KLK6) ELISA Kit | KLK6 elisa kit
Rat Kallikrein-6 (KLK6) ELISA Kit
Reactivity
Rat
Synonyms
Kallikrein-6 (KLK6); Rat Kallikrein-6 (KLK6) ELISA Kit; KLK6; Bssp; Klk7; MGC9355; NEUROSIN; PRSS18; PRSS9; SP59; ZYME; hK6; kallikrein 6 (neurosin; zyme); protease M; protease; serine; 18; 9; KLK6 elisa kit
Reactivity
Rat
Specificity
Rat Kallikrein-6 (KLK6) ELISA Kit has high sensitivity and excellent specificity for detection of Rat KLK6. No significant cross-reactivity or interference between Rat KLK6 and analogues was observed.
Sequence Length
261
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Kallikrein-6 (KLK6) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Kallikrein-6 (KLK6) Standard Curve.)
Related Product Information for KLK6 elisa kit
Description: This Rat Kallikrein-6 (KLK6) ELISA Kit employs a two-site sandwich ELISA to quantitate KLK6 in samples. An antibody specific for KLK6 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any KLK6 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for KLK6 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of KLK6 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Kallikrein 6, also known as Zyme, Neurosin, PRSS9, myelencephalon-specific protease (MSP) and protease M, is a trypsin-like serine proteinase. Kallikrein 6 was originally described from the brain as an enzyme involved in degradation of amyloid plaque protein (APP) and hK6 was thought to be a beta secretase. Kallikrein 6 was shown to be elevated in the sera patients with Alzheimer's disease and Parkinson's disease and in animal models of multiple sclerosis. The neuronal protein alpha-synuclein was shown to be cleaved by hK6, as has APP and hK6 has been implicated in vascular morphogenesis and angiogenesis. MSP has also been shown to rapidly degrade myelin-specific protein, laminin and fibronectin.
Background: Kallikrein 6, also known as Zyme, Neurosin, PRSS9, myelencephalon-specific protease (MSP) and protease M, is a trypsin-like serine proteinase. Kallikrein 6 was originally described from the brain as an enzyme involved in degradation of amyloid plaque protein (APP) and hK6 was thought to be a beta secretase. Kallikrein 6 was shown to be elevated in the sera patients with Alzheimer's disease and Parkinson's disease and in animal models of multiple sclerosis. The neuronal protein alpha-synuclein was shown to be cleaved by hK6, as has APP and hK6 has been implicated in vascular morphogenesis and angiogenesis. MSP has also been shown to rapidly degrade myelin-specific protein, laminin and fibronectin.
NCBI and Uniprot Product Information
NCBI GI #
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
prostatic glandular kallikrein-6
UniProt Protein Name
Prostatic glandular kallikrein-6
Protein Family
UniProt Gene Name
Klk6
UniProt Synonym Gene Names
Klk-8; Klk8; rGK-8
UniProt Entry Name
KLK6_RAT
Uniprot Description
Function: Glandular kallikreins cleave Met-Lys and Arg-Ser bonds in kininogen to release Lys-bradykinin.
Catalytic activity: Preferential cleavage of Arg-|-Xaa bonds in small molecule substrates. Highly selective action to release kallidin (lysyl-bradykinin) from kininogen involves hydrolysis of Met-|-Xaa or Leu-|-Xaa.
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Product Notes
The Rat KLK6 klk6 (Catalog #AAA9712387) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9712387 ELISA Kit recognizes Rat KLK6. It is sometimes possible for the material contained within the vial of "Kallikrein-6 (KLK6), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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