Human Kallikrein-6 ELISA Kit | KLK6 elisa kit

Human Kallikrein-6 ELISA Kit

Cat. Num. AAA762061

• Gene Names: KLK6; hK6; Bssp; Klk7; SP59; PRSS9; PRSS18
• Reactivity: Human
• Synonyms: Kallikrein-6; Human Kallikrein-6 ELISA Kit; KLK6/Kallikrein-6/Bssp/PRSS18/PRSS9/SP59/hK6/ZYME; KLK6 elisa kit

• Reactivity: Human
• Sequence Length: 244

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 0.313-20ng/ml
• Sensitivity: 0.188ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for KLK6 elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Human KLK6 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human KLK6 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human KLK6 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Human KLK6 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 61744424
• NCBI GeneID: 5653
• NCBI Accession #: NP_001012982.1
• NCBI GenBank Nucleotide #: NM_001012964.2
• Molecular Weight: 4,333 Da
• NCBI Official Full Name: kallikrein-6 isoform A preproprotein
• NCBI Official Synonym Full Names: kallikrein related peptidase 6
• NCBI Official Symbol: KLK6
• NCBI Official Synonym Symbols: hK6; Bssp; Klk7; SP59; PRSS9; PRSS18
• NCBI Protein Information: kallikrein-6
• UniProt Protein Name: Kallikrein-6
• Protein Family: Kallikrein
• UniProt Gene Name: KLK6
• UniProt Synonym Gene Names: PRSS18; PRSS9
• UniProt Entry Name: KLK6_HUMAN

NCBI Description

This gene encodes a member of the kallikrein subfamily of the peptidase S1 family of serine proteases. Growing evidence suggests that many kallikreins are implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers. The encoded preproprotein is proteolytically processed to generate the mature protease. Expression of this protease is regulated by steroid hormones and may be elevated in multiple human cancers and in serum from psoriasis patients. The encoded protease may participate in the cleavage of amyloid precursor protein and alpha-synuclein, thus implicating this protease in Alzheimer's and Parkinson's disease, respectively. This gene is located in a gene cluster on chromosome 19. Alternative splicing results in multiple transcript variants, at least one of which encodes an isoform that is proteolytically processed. [provided by RefSeq, Feb 2016]

Uniprot Description

KLK6: Serine protease which exhibits a preference for Arg over Lys in the substrate P1 position and for Ser or Pro in the P2 position. Shows activity against amyloid precursor protein, myelin basic protein, gelatin, casein and extracellular matrix proteins such as fibronectin, laminin, vitronectin and collagen. Degrades alpha-synuclein and prevents its polymerization, indicating that it may be involved in the pathogenesis of Parkinson disease and other synucleinopathies. May be involved in regulation of axon outgrowth following spinal cord injury. Tumor cells treated with a neutralizing KLK6 antibody migrate less than control cells, suggesting a role in invasion and metastasis. Belongs to the peptidase S1 family. Kallikrein subfamily. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Protease; Nucleolus; EC 3.4.21.-; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 19q13.3

Cellular Component: cytoplasm; endoplasmic reticulum; extracellular region; extracellular space; intercellular bridge; microtubule cytoskeleton; mitochondrion; nuclear membrane; nucleolus; nucleoplasm

Molecular Function: protein binding; serine-type endopeptidase activity

Biological Process: amyloid precursor protein metabolic process; central nervous system development; collagen catabolic process; hormone metabolic process; myelination; positive regulation of G-protein coupled receptor protein signaling pathway; protein autoprocessing; protein processing; regulation of cell differentiation; response to wounding; tissue regeneration