Human Kallikrein 6 (KLK6) ELISA Kit | KLK6 elisa kit

Human Kallikrein 6 (KLK6) ELISA Kit

Cat. Num. AAA2702286

• Gene Names: KLK6; hK6; Bssp; Klk7; SP59; PRSS9; PRSS18
• Reactivity: Human
• Synonyms: Kallikrein 6 (KLK6); Human Kallikrein 6 (KLK6) ELISA Kit; ZYME; Bssp; Klk7; Neurosin; Zyme; PRSS18; PRSS9; SP59; HK6; Protease M; Serine protease 18; KLK6 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Kallikrein 6 (KLK6).; No significant cross-reactivity or interference between Kallikrein 6 (KLK6) and analogues was observed.
• Sequence Length: 137

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma, Tissue Homogenates, Cerebrospinal Fluid, Breast Milk and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.059ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Kallikrein 6 (KLK6) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Kallikrein 6 (KLK6) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for KLK6 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Kallikrein 6 (KLK6). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Kallikrein 6 (KLK6). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Kallikrein 6 (KLK6), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Kallikrein 6 (KLK6) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 987437908
• NCBI GeneID: 5653
• NCBI Accession #: NP_001306878.1
• NCBI GenBank Nucleotide #: NM_001319949.1
• UniProt Accession #: Q92876; Q6H301; A6NJA1; A8MW09
• Molecular Weight: 4,333 Da
• NCBI Official Full Name: kallikrein-6 isoform B
• NCBI Official Synonym Full Names: kallikrein related peptidase 6
• NCBI Official Symbol: KLK6
• NCBI Official Synonym Symbols: hK6; Bssp; Klk7; SP59; PRSS9; PRSS18
• NCBI Protein Information: kallikrein-6
• UniProt Protein Name: Kallikrein-6
• Protein Family: Kallikrein
• UniProt Gene Name: KLK6
• UniProt Synonym Gene Names: PRSS18; PRSS9

NCBI Description

This gene encodes a member of the kallikrein subfamily of the peptidase S1 family of serine proteases. Growing evidence suggests that many kallikreins are implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers. The encoded preproprotein is proteolytically processed to generate the mature protease. Expression of this protease is regulated by steroid hormones and may be elevated in multiple human cancers and in serum from psoriasis patients. The encoded protease may participate in the cleavage of amyloid precursor protein and alpha-synuclein, thus implicating this protease in Alzheimer's and Parkinson's disease, respectively. This gene is located in a gene cluster on chromosome 19. Alternative splicing results in multiple transcript variants, at least one of which encodes an isoform that is proteolytically processed. [provided by RefSeq, Feb 2016]

Uniprot Description

KLK6: Serine protease which exhibits a preference for Arg over Lys in the substrate P1 position and for Ser or Pro in the P2 position. Shows activity against amyloid precursor protein, myelin basic protein, gelatin, casein and extracellular matrix proteins such as fibronectin, laminin, vitronectin and collagen. Degrades alpha-synuclein and prevents its polymerization, indicating that it may be involved in the pathogenesis of Parkinson disease and other synucleinopathies. May be involved in regulation of axon outgrowth following spinal cord injury. Tumor cells treated with a neutralizing KLK6 antibody migrate less than control cells, suggesting a role in invasion and metastasis. Belongs to the peptidase S1 family. Kallikrein subfamily. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.4.21.-; Nucleolus; Protease; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 19q13.41

Cellular Component: cytoplasm; endoplasmic reticulum; extracellular region; extracellular space; mitochondrion; nucleolus; secretory granule

Molecular Function: protein binding; serine-type endopeptidase activity

Biological Process: amyloid precursor protein metabolic process; central nervous system development; collagen catabolic process; hormone metabolic process; myelination; positive regulation of G-protein coupled receptor protein signaling pathway; protein autoprocessing; regulation of cell differentiation; response to wounding; tissue regeneration