Rat Hepcidin (Hepc) ELISA Kit | Hepc elisa kit

Rat Hepcidin (Hepc) ELISA Kit

Cat. Num. AAA450611

• Gene Names: Hamp; Hepc
• Reactivity: Rat
• Synonyms: Hepcidin (Hepc); Rat Hepcidin (Hepc) ELISA Kit; HAMP; HFE2B; PLTR; LEAP1; Hepcidin Antimicrobial Peptide; Liver-expressed antimicrobial peptide 1; Putative liver tumor regressor; Hepc elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of Hepc. No significant cross-reactivity or interference between Hepc and analogues was observed.
• Sequence Length: 84

• Samples: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
• Assay Type: Sandwich
• Detection Range: 0.625-40ng/mL
• Sensitivity: 0.227ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Hepc were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Hepc were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for Hepc elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of Hepc in Rat serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to Hepc. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to Hepc. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Hepc, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Hepc in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 16758218
• NCBI GeneID: 84604
• NCBI Accession #: NP_445921.1
• NCBI GenBank Nucleotide #: NM_053469.1
• UniProt Accession #: Q99MH3
• Molecular Weight: 9,286 Da
• NCBI Official Full Name: hepcidin
• NCBI Official Synonym Full Names: hepcidin antimicrobial peptide
• NCBI Official Symbol: Hamp
• NCBI Official Synonym Symbols: Hepc
• NCBI Protein Information: hepcidin
• UniProt Protein Name: Hepcidin
• Protein Family: Heparin-sulfate lyase
• UniProt Gene Name: Hamp
• UniProt Synonym Gene Names: Hepc

NCBI Description

may play a role in the regulation of iron metabolism [RGD, Feb 2006]

Uniprot Description

Seems to act as a signaling molecule involved in the maintenance of iron homeostasis. Seems to be required in conjunction with HFE to regulate both intestinal iron absorption and iron storage in macrophages. May also have antimicrobial activity ().