Mouse CD1c Monoclonal Antibody | anti-CD1c antibody

anti-human CD1c-Purified (with Antibiotics)

Cat. Num. AAA666365

• Gene Names: CD1C; R7; CD1; CD1A; BDCA1
• Reactivity: Human, Rabbit
• Applications: Flow Cytometry, Functional Assay, Immunohistochemistry
• Purity: Purified (with Antibiotics)
• Synonyms: CD1c; Monoclonal Antibody; anti-human CD1c-Purified (with Antibiotics); Monoclonal anti-human CD1c; anti-CD1c antibody

• Host: Mouse
• Reactivity: Human, Rabbit
• Clonality: Monoclonal
• Isotype: IgG1k
• Clone Number: M241
• Purity/Purification: Purified (with Antibiotics)
• Form/Format: 50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl, 0.5% Gentamicin sulfate
• Concentration: 1.0 mg/ml (varies by lot)
• Sequence Length: 333

• Applicable Applications for anti-CD1c antibody: Flow Cytometry (FC/FACS), Immunohistochemistry (IHC) (FS)
• Performance: Five x 105 cultured human Molt-4 cells per tube were incubated 45 minutes on ice with 80 ul anti-CD1c antibody at 10 ug/ml. Cells were washed twice and incubated with 2 degree reagent Goat anti-Mouse IgG/FITC, after which they were washed three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 1.45 log10 fluorescent units when compared to a Mouse IgG1 negative control at a similar concentration.
• Production: Antibody was Protein A purified from (low FBS containing) tissue culture supernatant. Purity was >95% Immunoglobulin by SDS-PAGE with less than 1% Bovine Immunoglobulin.
• Buffer: 50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl, 0.5 mg/ml Gentamicin Sulfate (as a preservative).
• Group Header: anti-human CD1c
• Immunogen: Human T cell line Molt 4
• Preparation and Storage: Store at 2 - 5 degree C. Freeze/Thawing is not recommended.; Product should retain activity for at least 12 months after shipping date when stored as recommended.

Testing Data

Related Product Information for anti-CD1c antibody

Information: The human CD1 genetic locus consists of five closely related genes. Three of the gene products (CD1a, CD1b and CD1c) have been officially clustered using antibodies and are expressed on cortical thymocytes, a subset of B cells, and on most professional antigen presenting cells. CD1 molecules have a specialized lipid antigen-presenting function and it is unlikely that they present conventional peptide antigens. CD1 may also be involved in costimulation.Antibody M241 recognizes the CD1c molecule of about 48 kd.

References

R.W. Knowles and W. F. Bodmer (1982) Eur J Immunol 12: 676-681. Leukocyte Typing III (A.J. McMichael, et al, eds.) Oxford University Press, Oxford, (1987) p. 882. Leukocyte Typing V (S.F. Schlossman, et al, eds.) Oxford University Press, Oxford, (1995) p. 337-341. E.M. Beckman, et al, (1994) Nature 372: 691-694. S. Blumberg, et al, (1995) Immunol Rev 147: 5-29. S.M. Behar, et al, (1995) J Exp Med 182: 2007-2018.

NCBI and Uniprot Product Information

• NCBI GI #: 48146277
• NCBI GeneID: 911
• UniProt Accession #: P29017; Q5TDJ7; Q6IAS4; Q9UMM0; Q9UN96
• Molecular Weight: 37,654 Da
• NCBI Official Full Name: CD1C
• NCBI Official Synonym Full Names: CD1c molecule
• NCBI Official Symbol: CD1C
• NCBI Official Synonym Symbols: R7; CD1; CD1A; BDCA1
• NCBI Protein Information: T-cell surface glycoprotein CD1c; CD1C antigen, c polypeptide; cortical thymocyte antigen CD1C; differentiation antigen CD1-alpha-3
• UniProt Protein Name: T-cell surface glycoprotein CD1c
• Protein Family: T-cell surface glycoprotein
• UniProt Gene Name: CD1C
• UniProt Entry Name: CD1C_HUMAN

NCBI Description

This gene encodes a member of the CD1 family of transmembrane glycoproteins, which are structurally related to the major histocompatibility complex (MHC) proteins and form heterodimers with beta-2-microglobulin. The CD1 proteins mediate the presentation of primarily lipid and glycolipid antigens of self or microbial origin to T cells. The human genome contains five CD1 family genes organized in a cluster on chromosome 1. The CD1 family members are thought to differ in their cellular localization and specificity for particular lipid ligands. The protein encoded by this gene is broadly distributed throughout the endocytic system via a tyrosine-based motif in the cytoplasmic tail. Alternatively spliced transcript variants of this gene have been observed, but their full-length nature is not known. [provided by RefSeq, Jul 2008]

Uniprot Description

CD1C: Antigen-presenting protein that binds self and non-self lipid and glycolipid antigens and presents them to T-cell receptors on natural killer T-cells.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 1q23.1

Cellular Component: integral to plasma membrane; plasma membrane; endosome membrane

Molecular Function: beta-2-microglobulin binding; exogenous lipid antigen binding; endogenous lipid antigen binding; glycolipid binding

Biological Process: antigen processing and presentation, exogenous lipid antigen via MHC class Ib; T cell activation during immune response