Mouse Hepcidin (Hepc) RTU ELISA Kit | Hepc rtu elisa kit

Mouse Hepcidin (Hepc) ELISA Kit

Cat. Num. AAA459835

• Gene Names: Hamp; Hepc; Hamp1; Hepc1
• Reactivity: Mouse
• Synonyms: Hepcidin (Hepc); Mouse Hepcidin (Hepc) ELISA Kit; HAMP; HFE2B; PLTR; LEAP1; Hepcidin Antimicrobial Peptide; Liver-expressed antimicrobial peptide 1; Putative liver tumor regressor; Hepc rtu elisa kit

• Reactivity: Mouse
• Sequence Length: 83

• Assay Type: Sandwich
• Samples: Serum, Plasma, Urine or Other Biological Fluids.
• Detection Range: 62.5-4000pg/mL
• Sensitivity: 25.4pg/mL
• RTU ELISA Kit's Advantage: The Ready-To-Use (RTU) Kits require fewer steps in setup and take less time to run. Detection Solutions are pre-diluted and total incubation time is shorter than many other ELISA Kits.
• Preparation and Storage: For unopened kits: All the reagents should be kept at 4 degree C upon receipt.; For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for Hepc rtu elisa kit

Test Principle: The microtiter plate provided in this kit has been pre-coated with an antibody specific to Hepc. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to Hepc. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Hepc, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Hepc in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 14211542
• NCBI GeneID: 84506
• NCBI Accession #: NP_115930.1
• NCBI GenBank Nucleotide #: NM_032541.2
• UniProt Accession #: Q9EQ21
• Molecular Weight: 9,352 Da
• NCBI Official Full Name: hepcidin preproprotein
• NCBI Official Synonym Full Names: hepcidin antimicrobial peptide
• NCBI Official Symbol: Hamp
• NCBI Official Synonym Symbols: Hepc; Hamp1; Hepc1
• NCBI Protein Information: hepcidin
• UniProt Protein Name: Hepcidin
• Protein Family: Heparin-sulfate lyase
• UniProt Gene Name: Hamp
• UniProt Synonym Gene Names: Hamp1; Hepc; Hepc1

NCBI Description

This gene encodes hepcidin, an antimicrobial peptide and master hormonal regulator of systemic iron metabolism. The encoded preproprotein is synthesized in the hepatocytes where it undergoes proteolytic processing to generate disulfide-linked mature peptides that are secreted into the bloodstream. Mice lacking the encoded protein develop multivisceral iron overlaod, with sparing of the spleen macrophages. Certain mutations in the human ortholog of this gene cause hemochromatosis type 2B, also known as juvenile hemochromatosis. This gene is located adjacent to a related hepcidin gene on chromosome 7. [provided by RefSeq, Aug 2016]

Uniprot Description

Liver-produced hormone that constitutes the main circulating regulator of iron absorption and distribution across tissues. Acts by promoting endocytosis and degradation of ferroportin, leading to the retention of iron in iron-exporting cells and decreased flow of iron into plasma. Controls the major flows of iron into plasma: absorption of dietary iron in the intestine, recycling of iron by macrophages, which phagocytose old erythrocytes and other cells, and mobilization of stored iron from hepatocytes.