Rat GNb1 ELISA Kit | GNb1 elisa kit

Rat GNb1 (G Protein Beta 1) ELISA Kit

Cat. Num. AAA2508344

• Reactivity: Rat
• Synonyms: GNb1; Rat GNb1 (G Protein Beta 1) ELISA Kit; GNb1 elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of Rat GNb1. No significant cross-reactivity or interference between Rat GNb1 and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Rat GNb1 and all the analogues, therefore, cross reaction may still exist.
• Sequence Length: 340

• Samples: Serum, Plasma, Biological Fluids
• Assay Type: Sandwich
• Detection Range: 15.625-1000?IU/mL
• Sensitivity: Min: 9.375?IU/mL; Max: 1000?IU/mL
• Preparation and Storage: Store at 4 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for GNb1 elisa kit

Description: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of GNb1 in Rat serum, plasma and other biological fluids
Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat GNb1. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Rat GNb1 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat GNb1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Rat GNb1. You can calculate the concentration of Rat GNb1 in the samples by comparing the OD of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 543173169
• NCBI GeneID: 2782
• NCBI Accession #: NP_001269468.1
• NCBI GenBank Nucleotide #: NM_001282539.1
• UniProt Accession #: P62873; P04697; P04901; Q1RMY8; B1AJZ7
• Molecular Weight: 36,298 Da
• NCBI Official Full Name: guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 isoform 1
• NCBI Official Synonym Full Names: guanine nucleotide binding protein (G protein), beta polypeptide 1
• NCBI Official Symbol: GNB1
• NCBI Protein Information: guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1; guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1; G protein, beta-1 subunit; beta subunit, signal-transducing proteins GS/GI; transducin beta chain 1
• UniProt Protein Name: Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1
• UniProt Gene Name: GNB1
• UniProt Entry Name: GBB1_HUMAN

NCBI Description

Heterotrimeric guanine nucleotide-binding proteins (G proteins), which integrate signals between receptors and effector proteins, are composed of an alpha, a beta, and a gamma subunit. These subunits are encoded by families of related genes. This gene encodes a beta subunit. Beta subunits are important regulators of alpha subunits, as well as of certain signal transduction receptors and effectors. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2013]

Uniprot Description

G-beta 1: Guanine nucleotide-binding proteins (G proteins) are involved as a modulator or transducer in various transmembrane signaling systems. The beta and gamma chains are required for the GTPase activity, for replacement of GDP by GTP, and for G protein- effector interaction. Belongs to the WD repeat G protein beta family.

Protein type: G protein, heterotrimeric beta; G protein, heterotrimeric; G protein

Chromosomal Location of Human Ortholog: 1p36.33

Cellular Component: photoreceptor inner segment; membrane; lysosomal membrane; dendrite; plasma membrane; heterotrimeric G-protein complex; intracellular; vesicle

Molecular Function: GTPase activity; signal transducer activity; protein binding; GTPase binding; spectrin binding; protein complex binding; type 1 angiotensin receptor binding

Biological Process: platelet activation; phototransduction, visible light; acetylcholine receptor signaling, muscarinic pathway; sensory perception of taste; signal transduction; rhodopsin mediated signaling; cell proliferation; synaptic transmission; elevation of cytosolic calcium ion concentration; regulation of rhodopsin mediated signaling; retina development in camera-type eye; Ras protein signal transduction; energy reserve metabolic process; G-protein signaling, coupled to IP3 second messenger (phospholipase C activating); blood coagulation; dopamine receptor, adenylate cyclase activating pathway