ABHD14B recombinant protein

ABHD14B Protein, Human, Recombinant (His Tag)

Cat. Num. AAA8121576

• Gene Names: ABHD14B; CIB
• Purity: >96% as determined by SDS-PAGE
• Synonyms: ABHD14B; ABHD14B Protein; Human; Recombinant (His Tag); Human ABHD14B Protein (His Tag); CIB Protein; HEL-S-299 Protein; abhydrolase domain containing 14B; ABHD14B recombinant protein

• Host: E Coli
• Purity/Purification: >96% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, 20% glycerol, 50mM Arg, 0.1% Tween20, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Gln210

• Species: Human
• Predicted N Terminal: His
• Tag: N-His
• Protein Construction: A DNA sequence encoding the mature form of human ABHD14B (Q96IU4-1)(Met1-Gln210) was expressed with a polyhistide tag at the N-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for ABHD14B recombinant protein

Background: ABHD14B belongs to the AB hydrolase superfamily, ABHD14 family. It can be detected in spleen, thymus, prostate, testis, ovary, small intestine, colon, peripheral blood leukocyte, heart, placenta, lung, liver, skeletal muscle, pancreas and kidney. ABHD14B has hydrolase activity towards p-nitrophenyl butyrate (in vitro) and may interact with TAF1. It may activate transcription. Recombinant human ABHD14B protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. ABHD14B contains an alpha/beta hydrolase fold, which is a catalytic domain found in a very wide range of enzymes. In molecular biology, the alpha/beta hydrolase fold is common to a number of hydrolytic enzymes of widely differing phylogenetic origin and catalytic function. The Ab hydrolase domain containing gene subfamily is comprised of 15 mostly uncharacterized members.

References

Mehrle A, et al. (2006) The LIFEdb database in 2006. Nucleic Acids Res. 34:D415-8.Wan D, et al. (2004) Large-scale cDNA transfection screening for genes related to cancer development and progression. Proc Natl Acad Sci. 101(44):15724-9.Wiemann S, et al. (2004) From ORFeome to biology: a functional genomics pipeline. Genome Res. 14 (10B):2136-44.

NCBI and Uniprot Product Information

• NCBI GI #: 226443152
• NCBI GeneID: 84836
• NCBI Accession #: NP_001139786.1
• NCBI GenBank Nucleotide #: NM_001146314.1
• UniProt Accession #: Q96IU4; Q86VK8; Q8N8W5
• Molecular Weight: 22,346 Da
• NCBI Official Full Name: alpha/beta hydrolase domain-containing protein 14B isoform 1
• NCBI Official Synonym Full Names: abhydrolase domain containing 14B
• NCBI Official Symbol: ABHD14B
• NCBI Official Synonym Symbols: CIB
• NCBI Protein Information: alpha/beta hydrolase domain-containing protein 14B; CCG1-interacting factor B; cell cycle gene 1-interacting factor B; abhydrolase domain-containing protein 14B
• UniProt Protein Name: Alpha/beta hydrolase domain-containing protein 14B
• Protein Family: Protein
• UniProt Gene Name: ABHD14B
• UniProt Synonym Gene Names: CIB; Abhydrolase domain-containing protein 14B
• UniProt Entry Name: ABHEB_HUMAN

Uniprot Description

ABHD14B: Has hydrolase activity towards p-nitrophenyl butyrate (in vitro). May activate transcription. Belongs to the AB hydrolase superfamily. ABHD14 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.-.-.-; Hydrolase

Chromosomal Location of Human Ortholog: 3p21.2

Cellular Component: cytoplasm; nucleolus; nucleus; cytosol

Molecular Function: protein binding; hydrolase activity

Biological Process: metabolic process; positive regulation of transcription from RNA polymerase II promoter