Rat Platelet-derived growth factor subunit B ELISA Kit | PDGFB elisa kit

Rat Platelet-derived growth factor subunit B ELISA Kit

Cat. Num. AAA763789

• Gene Names: PDGFB; SIS; SSV; IBGC5; PDGF2; c-sis; PDGF-2
• Reactivity: Rat
• Synonyms: Platelet-derived growth factor subunit B; Rat Platelet-derived growth factor subunit B ELISA Kit; PDGFB; B chain/Becaplermin/FLJ12858/PDGF subunit B/PDGF2/PDGF-2/platelet-derived growth factor 2/Platelet-derived growth factor beta polypeptide/platelet-derived growth factor subunit B/platelet-derived growth factor; B chain; PDGFB elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of PCT. No significant cross-reactivity or interference between PCT and analogues was observed.
• Sequence Length: 241

• Samples: Serum, Plasma, Tissue Homogenates And Other Biological Fluids
• Assay Type: Sandwich
• Detection Range: 31.25-2000pg/ml
• Sensitivity: <18.75pg/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level PCT were tested 20 times on one plate, respectively. Intra-Assay: CV<8%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level PCT were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for PDGFB elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-PCT antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-PCT antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the PCT amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of PCT can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 4505681
• NCBI GeneID: 5155
• NCBI Accession #: NP_002599.1
• NCBI GenBank Nucleotide #: NM_002608.3
• Molecular Weight: 25,502 Da
• NCBI Official Full Name: platelet-derived growth factor subunit B isoform 1 preproprotein
• NCBI Official Synonym Full Names: platelet derived growth factor subunit B
• NCBI Official Symbol: PDGFB
• NCBI Official Synonym Symbols: SIS; SSV; IBGC5; PDGF2; c-sis; PDGF-2
• NCBI Protein Information: platelet-derived growth factor subunit B
• UniProt Protein Name: Platelet-derived growth factor subunit B
• Protein Family: Platelet-derived growth factor
• UniProt Gene Name: PDGFB
• UniProt Synonym Gene Names: PDGF2; SIS; PDGF subunit B
• UniProt Entry Name: PDGFB_HUMAN

NCBI Description

This gene encodes a member of the protein family comprised of both platelet-derived growth factors (PDGF) and vascular endothelial growth factors (VEGF). The encoded preproprotein is proteolytically processed to generate platelet-derived growth factor subunit B, which can homodimerize, or alternatively, heterodimerize with the related platelet-derived growth factor subunit A. These proteins bind and activate PDGF receptor tyrosine kinases, which play a role in a wide range of developmental processes. Mutations in this gene are associated with meningioma. Reciprocal translocations between chromosomes 22 and 17, at sites where this gene and that for collagen type 1, alpha 1 are located, are associated with dermatofibrosarcoma protuberans, a rare skin tumor. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2015]

Uniprot Description

PDGFB: Growth factor that plays an essential role in the regulation of embryonic development, cell proliferation, cell migration, survival and chemotaxis. Potent mitogen for cells of mesenchymal origin. Required for normal proliferation and recruitment of pericytes and vascular smooth muscle cells in the central nervous system, skin, lung, heart and placenta. Required for normal blood vessel development, and for normal development of kidney glomeruli. Plays an important role in wound healing. Signaling is modulated by the formation of heterodimers with PDGFA. A chromosomal aberration involving PDGFB is found in dermatofibrosarcoma protuberans. Translocation t(17;22)(q22;q13) with PDGFB. Belongs to the PDGF/VEGF growth factor family.

Protein type: Motility/polarity/chemotaxis; Oncoprotein; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 22q13.1

Cellular Component: basolateral plasma membrane; cell surface; cytoplasm; endoplasmic reticulum lumen; extracellular matrix; extracellular region; extracellular space; Golgi membrane

Molecular Function: chemoattractant activity; collagen binding; growth factor activity; identical protein binding; phosphatidylinositol-4,5-bisphosphate 3-kinase activity; platelet-derived growth factor binding; platelet-derived growth factor receptor binding; protein binding; protein heterodimerization activity; protein homodimerization activity; Ras guanyl-nucleotide exchange factor activity; superoxide-generating NADPH oxidase activator activity

Biological Process: activation of protein kinase activity; activation of protein kinase B; embryonic placenta development; extracellular matrix organization and biogenesis; heart development; hemopoiesis; MAPKKK cascade; monocyte chemotaxis; negative regulation of phosphatidylinositol biosynthetic process; negative regulation of protein binding; negative regulation of transcription, DNA-dependent; peptidyl-serine phosphorylation; peptidyl-tyrosine phosphorylation; phosphoinositide-mediated signaling; platelet degranulation; platelet-derived growth factor receptor signaling pathway; positive regulation of blood vessel endothelial cell migration; positive regulation of cell migration; positive regulation of cell proliferation; positive regulation of chemotaxis; positive regulation of cyclin-dependent protein kinase activity; positive regulation of DNA replication; positive regulation of endothelial cell proliferation; positive regulation of fibroblast proliferation; positive regulation of glomerular filtration; positive regulation of MAP kinase activity; positive regulation of MAPKKK cascade; positive regulation of mitosis; positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of phosphoinositide 3-kinase activity; positive regulation of phosphoinositide 3-kinase cascade; positive regulation of protein amino acid autophosphorylation; positive regulation of smooth muscle cell migration; positive regulation of smooth muscle cell proliferation; positive regulation of transcription, DNA-dependent; protein amino acid phosphorylation; regulation of phosphoinositide 3-kinase cascade; response to wounding; transforming growth factor beta receptor signaling pathway

Disease: Basal Ganglia Calcification, Idiopathic, 1; Basal Ganglia Calcification, Idiopathic, 5; Dermatofibrosarcoma Protuberans; Meningioma, Familial, Susceptibility To