Rat Eotaxin 1 ELISA Kit | EOTAXIN 1 elisa kit

Rat Eotaxin 1 ELISA Kit

Cat. Num. AAA31966

• Gene Names: CCL11; SCYA11
• Reactivity: Rat
• Synonyms: Eotaxin 1; Rat Eotaxin 1 ELISA Kit; EOTAXIN 1 elisa kit

• Reactivity: Rat
• Specificity: No significant cross-reactivity or interference between Rat EOTAXIN 1 and analogues was observed.
• Sequence Length: 97

• Samples: Serum, Plasma, Tissue Homogenate, Feces, Urine and Body Fluids
• Assay Type: Sandwich
• Detection Range: 125 pg/ml - 4000 pg/ml.
• Sensitivity: 10 pg/ml.
• Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic applications! It is intended to be determinated EOTAXIN 1 concentrations in Rat serum, plasma and other body fluids. Using Purified Rat EOTAXIN 1 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add EOTAXIN 1 and EOTAXIN 1 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of EOTAXIN 1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• Intra-assay Precision: Intra-assay CV (%) is less than 15%.
• Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]
• Preparation and Storage: Store all reagents at 2-8 degree C

Related Product Information for EOTAXIN 1 elisa kit

Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only! It is intended to be determinated EOTAXIN 1 concentrations in Rat serum, plasma and other body fluids. Using Purified Rat EOTAXIN 1 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add EOTAXIN 1 and EOTAXIN 1 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of EOTAXIN 1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 4506827
• NCBI GeneID: 6356
• NCBI Accession #: NP_002977.1
• NCBI GenBank Nucleotide #: NM_002986.2
• UniProt Accession #: P51671; P50877; Q92490; Q92491
• Molecular Weight: 10,732 Da
• NCBI Official Full Name: eotaxin
• NCBI Official Synonym Full Names: chemokine (C-C motif) ligand 11
• NCBI Official Symbol: CCL11
• NCBI Official Synonym Symbols: SCYA11
• NCBI Protein Information: eotaxin; eotaxin-1; eosinophil chemotactic protein; small inducible cytokine subfamily A (Cys-Cys), member 11 (eotaxin)
• UniProt Protein Name: Eotaxin
• UniProt Gene Name: CCL11
• UniProt Synonym Gene Names: SCYA11
• UniProt Entry Name: CCL11_HUMAN

NCBI Description

This gene is one of several chemokine genes clustered on the q-arm of chromosome 17. Chemokines form a superfamily of secreted proteins involved in immunoregulatory and inflammatory processes. The superfamily is divided into four subfamilies based on the arrangement of the N-terminal cysteine residues of the mature peptide. This chemokine, a member of the CC subfamily, displays chemotactic activity for eosinophils, but not mononuclear cells or neutrophils. This eosinophil-specific chemokine is thought to be involved in eosinophilic inflammatory diseases such as atopic dermatitis, allergic rhinitis, asthma and parasitic infections. [provided by RefSeq, Jul 2013]

Uniprot Description

CCL11: In response to the presence of allergens, this protein directly promotes the accumulation of eosinophils, a prominent feature of allergic inflammatory reactions. Binds to CCR3. Belongs to the intercrine beta (chemokine CC) family.

Protein type: Motility/polarity/chemotaxis; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 17q12

Cellular Component: extracellular space; extracellular region

Molecular Function: protein binding; chemokine activity

Biological Process: chronic inflammatory response; mast cell chemotaxis; response to virus; actin filament organization; cytoskeleton organization and biogenesis; chemotaxis; signal transduction; protein amino acid phosphorylation; cellular calcium ion homeostasis; regulation of cell shape; response to radiation; positive regulation of angiogenesis; positive regulation of actin filament polymerization; eosinophil chemotaxis; immune response; positive regulation of endothelial cell proliferation; cell adhesion; inflammatory response; positive regulation of cell migration

Disease: Asthma, Susceptibility To; Human Immunodeficiency Virus Type 1, Susceptibility To