Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat DNA-directed RNA polymerases I, II, and III subunit RPABC1 (POLR2E) Standard Curve.)
Rat DNA-Directed RNA Polymerases I, II, And III Subunit RPABC1 (POLR2E) ELISA Kit | POLR2E elisa kit
Rat DNA-Directed RNA Polymerases I, II, And III Subunit RPABC1 (POLR2E) ELISA Kit
Reactivity
Rat
Synonyms
DNA-Directed RNA Polymerases I; II; And III Subunit RPABC1 (POLR2E); Rat DNA-Directed RNA Polymerases I; And III Subunit RPABC1 (POLR2E) ELISA Kit; POLR2E; RPABC1; RPB5; XAP4; hRPB25; hsRPB5; DNA directed RNA polymerase 23 kda polypeptide; DNA directed RNA polymerase polypeptide E; POLR2E elisa kit
Reactivity
Rat
Specificity
Rat DNA-directed RNA polymerases I, II, and III subunit RPABC1 (POLR2E) ELISA Kit has high sensitivity and excellent specificity for detection of Rat POLR2E. No significant cross-reactivity or interference between Rat POLR2E and analogues was observed.
Sequence Length
210
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat DNA-directed RNA polymerases I, II, and III subunit RPABC1 (POLR2E) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat DNA-directed RNA polymerases I, II, and III subunit RPABC1 (POLR2E) Standard Curve.)
Related Product Information for POLR2E elisa kit
Description: This Rat DNA-directed RNA polymerases I, II, and III subunit RPABC1 (POLR2E) ELISA Kit employs a two-site sandwich ELISA to quantitate POLR2E in samples. An antibody specific for POLR2E has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any POLR2E present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for POLR2E is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of POLR2E bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: POLR2E encodes the fifth largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. This subunit is shared by the other two DNA-directed RNA polymerases and is present in two-fold molar excess over the other polymerase subunits. An interaction between this subunit and a hepatitis virus transactivating protein has been demonstrated, suggesting that interaction between transcriptional activators and the polymerase can occur through this subunit. A pseudogene is located on chromosome 11. A second site of hybridization to 11q23.1 was thought to represent a pseudogene, most likely a retroposon, since no remnants of introns could be identified in the study of genomic DNA.
Background: POLR2E encodes the fifth largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. This subunit is shared by the other two DNA-directed RNA polymerases and is present in two-fold molar excess over the other polymerase subunits. An interaction between this subunit and a hepatitis virus transactivating protein has been demonstrated, suggesting that interaction between transcriptional activators and the polymerase can occur through this subunit. A pseudogene is located on chromosome 11. A second site of hybridization to 11q23.1 was thought to represent a pseudogene, most likely a retroposon, since no remnants of introns could be identified in the study of genomic DNA.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
DNA-directed RNA polymerases I, II, and III subunit RPABC1
NCBI Official Synonym Full Names
RNA polymerase II subunit E
NCBI Official Symbol
Polr2e
NCBI Protein Information
DNA-directed RNA polymerases I, II, and III subunit RPABC1
UniProt Protein Name
DNA-directed RNA polymerases I, II, and III subunit RPABC1
UniProt Gene Name
Polr2e
UniProt Synonym Gene Names
RNA polymerases I, II, and III subunit ABC1
UniProt Entry Name
RPAB1_RAT
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Product Notes
The Rat POLR2E polr2e (Catalog #AAA9712553) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9712553 ELISA Kit recognizes Rat POLR2E. It is sometimes possible for the material contained within the vial of "DNA-Directed RNA Polymerases I, II, And III Subunit RPABC1 (POLR2E), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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