Western Blot (WB)
(POLR2E Antibody (N-term) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the POLR2E antibody detected the POLR2E protein (arrow).)
Rabbit POLR2E Polyclonal Antibody | anti-POLR2E antibody
POLR2E Antibody (N-term)
Western Blot (WB)
(POLR2E Antibody (N-term) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the POLR2E antibody detected the POLR2E protein (arrow).)
Western Blot (WB)
(POLR2E Antibody (N-term) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the POLR2E antibody detected the POLR2E protein (arrow).)
polymerase II, the polymerase responsible for synthesizing
messenger RNA in eukaryotes. This subunit is shared by the other
two DNA-directed RNA polymerases and is present in two-fold molar
excess over the other polymerase subunits. An interaction between
this subunit and a hepatitis virus transactivating protein has been
demonstrated, suggesting that interaction between transcriptional
activators and the polymerase can occur through this subunit. A
pseudogene is located on chromosome 11.
Cloutier, P., et al. Methods 48(4):381-386(2009)
Michiels, S., et al. Carcinogenesis 30(5):763-768(2009)
Lamesch, P., et al. Genomics 89(3):307-315(2007)
Le, T.T., et al. J. Biochem. 138(3):215-224(2005)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes the fifth largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. This subunit is shared by the other two DNA-directed RNA polymerases and is present in two-fold molar excess over the other polymerase subunits. An interaction between this subunit and a hepatitis virus transactivating protein has been demonstrated, suggesting that interaction between transcriptional activators and the polymerase can occur through this subunit. A pseudogene is located on chromosome 11. [provided by RefSeq, Jul 2008]
Uniprot Description
POLR2E: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Common component of RNA polymerases I, II and III which synthesize ribosomal RNA precursors, mRNA precursors and many functional non-coding RNAs, and small RNAs, such as 5S rRNA and tRNAs, respectively. Pol II is the central component of the basal RNA polymerase II transcription machinery. Pols are composed of mobile elements that move relative to each other. In Pol II, POLR2E/RPB5 is part of the lower jaw surrounding the central large cleft and thought to grab the incoming DNA template. Seems to be the major component in this process. Belongs to the archaeal RpoH/eukaryotic RPB5 RNA polymerase subunit family.
Chromosomal Location of Human Ortholog: 19p13.3
Cellular Component: nucleoplasm; DNA-directed RNA polymerase III complex; DNA-directed RNA polymerase II, core complex; cytosol; nucleus; DNA-directed RNA polymerase I complex
Molecular Function: protein binding; DNA binding; DNA-directed RNA polymerase activity
Biological Process: transcription from RNA polymerase II promoter; transcription initiation from RNA polymerase II promoter; termination of RNA polymerase III transcription; viral reproduction; positive regulation of viral transcription; somatic stem cell maintenance; RNA splicing; transcription from RNA polymerase I promoter; RNA elongation from RNA polymerase I promoter; DNA repair; termination of RNA polymerase I transcription; regulation of gene expression, epigenetic; transcription from RNA polymerase III promoter; nuclear mRNA splicing, via spliceosome; mRNA capping; negative regulation of gene expression, epigenetic; nucleotide-excision repair; transcription-coupled nucleotide-excision repair; RNA elongation from RNA polymerase II promoter; positive regulation of interferon type I production; innate immune response; gene expression; transcription initiation from RNA polymerase I promoter; RNA elongation from RNA polymerase III promoter
Research Articles on POLR2E
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Product Notes
The POLR2E polr2e (Catalog #AAA9206916) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 30-59. The POLR2E Antibody (N-term) reacts with Human (Predicted Reactivity: Mouse, Rat) and may cross-react with other species as described in the data sheet. AAA Biotech's POLR2E can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB~~1:1000. Researchers should empirically determine the suitability of the POLR2E polr2e for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "POLR2E, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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