Rabbit anti-Human, Mouse UBE2D1 / UBCH5 Polyclonal Antibody | anti-UBE2D1 antibody
Rabbit Polyclonal to Human UBE2D1 / UBCH5
Immunohistochemistry (IHC)
(Anti-UBE2D1 / UBCH5 antibody IHC staining of human skin, epidermis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)
Western Blot (WB)
(Western blot of anti-UBE2D1 Pab in, left-to-right, wild-type T3 cells (mouse mouse fibroblast cell line), wild-type T3 cells plus recombinant mouse UBE2D1(120 ng), and HeLa cells. Electrophoresis performed on a 10% Bis-Tris gel. Membrane blocking with 10% milk-PBST. Blotting at 1:500 dilution of primary antibody. Data courtesy of Xiaoli Wang, MD. PhD., Washington University School of Medicine.)
Western Blot (WB)
(Western blot of anti-UBE2D1 Pab in mouse cerebellum tissue lysates (35 ug/lane). UBE2D1 (arrow) was detected using the purified Pab.)
NCBI and Uniprot Product Information
NCBI Description
The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is closely related to a stimulator of iron transport (SFT), and is up-regulated in hereditary hemochromatosis. It also functions in the ubiquitination of the tumor-suppressor protein p53 and the hypoxia-inducible transcription factor HIF1alpha by interacting with the E1 ubiquitin-activating enzyme and the E3 ubiquitin-protein ligases. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2011]
Uniprot Description
UBE2D1: Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys- 48'-linked polyubiquitination. Mediates the selective degradation of short-lived and abnormal proteins. Functions in the E6/E6-AP- induced ubiquitination of p53/TP53. Mediates ubiquitination of PEX5 and auto-ubiquitination of STUB1, TRAF6 and TRIM63/MURF1. Ubiquitinates STUB1-associated HSP90AB1 in vitro. Lacks inherent specificity for any particular lysine residue of ubiquitin. Essential for viral activation of IRF3. Mediates polyubiquitination of CYP3A4. Belongs to the ubiquitin-conjugating enzyme family.
Protein type: Ligase; Ubiquitin ligase; EC 6.3.2.19; Ubiquitin conjugating system
Chromosomal Location of Human Ortholog: 10q21.1
Cellular Component: nucleoplasm; protein complex; cytoplasm; cytosol; ubiquitin ligase complex
Molecular Function: protein binding; ubiquitin-protein ligase activity; ATP binding; ligase activity
Biological Process: positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle; ubiquitin-dependent protein catabolic process; transcription initiation from RNA polymerase II promoter; negative regulation of ubiquitin-protein ligase activity during mitotic cell cycle; protein polyubiquitination; transcription, DNA-dependent; MyD88-independent toll-like receptor signaling pathway; toll-like receptor 3 signaling pathway; negative regulation of transcription from RNA polymerase II promoter; BMP signaling pathway; regulation of ubiquitin-protein ligase activity during mitotic cell cycle; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; positive regulation of protein ubiquitination; transforming growth factor beta receptor signaling pathway; mitotic cell cycle spindle assembly checkpoint; toll-like receptor signaling pathway; innate immune response; gene expression; mitotic cell cycle; toll-like receptor 4 signaling pathway
