Western Blot (WB)
(Western blot analysis of GRP78 expression in Hela (A), rat liver (B) whole cell lysates.)
Mouse anti-Human, Rat GRP78 Monoclonal Antibody | anti-HSPA5 antibody
Anti-GRP78 Antibody
Western Blot (WB)
(Western blot analysis of GRP78 expression in Hela (A), rat liver (B) whole cell lysates.)
Western Blot (WB)
(Western blot analysis of GRP78 expression in Hela (A), rat liver (B) whole cell lysates.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of GRP78 staining in human colon cancer,human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of GRP78 staining in human colon cancer,human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a member of the heat shock protein 70 (HSP70) family. It is localized in the lumen of the endoplasmic reticulum (ER), and is involved in the folding and assembly of proteins in the ER. As this protein interacts with many ER proteins, it may play a key role in monitoring protein transport through the cell.[provided by RefSeq, Sep 2010]
Uniprot Description
GRP78: a member of the HSP family of molecular chaperones required for endoplasmic reticulum integrity and stress-induced autophagy. Plays a central role in regulating the unfolded protein response (UPR), and is an obligatory component of autophagy in mammalian cells. May play an important role in cellular adaptation and oncogenic survival. One of the client proteins of GRP78 is protein double-stranded RNA-activated protein-like endoplasmic reticulum kinase (PERK). Probably plays a role in facilitating the assembly of multimeric protein complexes inside the ER.
Protein type: Chaperone; Heat shock protein
Chromosomal Location of Human Ortholog: 9q33.3
Cellular Component: cell surface; endoplasmic reticulum; endoplasmic reticulum lumen; endoplasmic reticulum membrane; ER-Golgi intermediate compartment; focal adhesion; integral to endoplasmic reticulum membrane; melanosome; membrane; midbody; mitochondrion; myelin sheath; nucleus; plasma membrane; signalosome; smooth endoplasmic reticulum
Molecular Function: ATP binding; ATPase activity; calcium ion binding; chaperone binding; enzyme binding; glycoprotein binding; misfolded protein binding; protein binding; protein domain specific binding; ribosome binding; ubiquitin protein ligase binding; unfolded protein binding
Biological Process: blood coagulation; cellular protein metabolic process; cellular response to glucose starvation; cerebellar Purkinje cell layer development; cerebellum structural organization; ER overload response; ER-associated protein catabolic process; negative regulation of apoptosis; negative regulation of transforming growth factor beta receptor signaling pathway; platelet activation; platelet degranulation; positive regulation of cell migration; positive regulation of protein ubiquitination; substantia nigra development; unfolded protein response; unfolded protein response, activation of signaling protein activity
Research Articles on HSPA5
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Product Notes
The HSPA5 hspa5 (Catalog #AAA8241533) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-GRP78 Antibody reacts with Human, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's GRP78 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). Western Blot: (1/1000 - 1/2000); Immunohistochemistry: (1/100 - 1/200). Researchers should empirically determine the suitability of the HSPA5 hspa5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GRP78, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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