Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Delta-like protein 4 (DLL4) Standard Curve.)
Mouse Delta-Like Protein 4 (DLL4) ELISA Kit | DLL4 elisa kit
Mouse Delta-Like Protein 4 (DLL4) ELISA Kit
Gene Names
Dll4; Delta4
Reactivity
Mouse
Synonyms
Delta-Like Protein 4 (DLL4); Mouse Delta-Like Protein 4 (DLL4) ELISA Kit; DLL4; MGC126344; hdelta2; delta 4; delta ligand 4; delta-like 4 homolog; delta-like 4 protein; notch ligand DLL4; notch ligand delta-2; DLL4 elisa kit
Reactivity
Mouse
Specificity
Mouse Delta-like protein 4 (DLL4) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse DLL4. No significant cross-reactivity or interference between Mouse DLL4 and analogues was observed.
Sequence Length
686
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Delta-like protein 4 (DLL4) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Delta-like protein 4 (DLL4) Standard Curve.)
Related Product Information for DLL4 elisa kit
Description: This Mouse Delta-like protein 4 (DLL4) ELISA Kit employs a two-site sandwich ELISA to quantitate DLL4 in samples. An antibody specific for DLL4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any DLL4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DLL4 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DLL4 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: DLL4 is a homolog of the Drosophila delta gene. The delta gene family encodes Notch ligands that are characterized by a DSL domain, EGF repeats, and a transmembrane domain. The predicted 685-amino acid DLL4 protein exhibits all of the hallmarks of the Delta family of Notch ligands. It has an extracellular region containing 8 EGF-like repeats and a DSL domain involved in Notch binding, as well as a transmembrane domain and a cytoplasmic tail that lacks catalytic motifs. Human DLL4 shares 87% amino acid sequence identity with mouse Dll4. Northern blot analysis of mouse tissues detected highest Dll4 expression in lung, followed by heart, kidney, skeletal muscle, and brain; Dll4 expression was barely detectable in spleen and testis.
Background: DLL4 is a homolog of the Drosophila delta gene. The delta gene family encodes Notch ligands that are characterized by a DSL domain, EGF repeats, and a transmembrane domain. The predicted 685-amino acid DLL4 protein exhibits all of the hallmarks of the Delta family of Notch ligands. It has an extracellular region containing 8 EGF-like repeats and a DSL domain involved in Notch binding, as well as a transmembrane domain and a cytoplasmic tail that lacks catalytic motifs. Human DLL4 shares 87% amino acid sequence identity with mouse Dll4. Northern blot analysis of mouse tissues detected highest Dll4 expression in lung, followed by heart, kidney, skeletal muscle, and brain; Dll4 expression was barely detectable in spleen and testis.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
delta-like protein 4
NCBI Official Synonym Full Names
delta like canonical Notch ligand 4
NCBI Official Symbol
Dll4
NCBI Official Synonym Symbols
Delta4
NCBI Protein Information
delta-like protein 4
UniProt Protein Name
Delta-like protein 4
Protein Family
UniProt Gene Name
Dll4
UniProt Synonym Gene Names
Delta4
UniProt Entry Name
DLL4_MOUSE
Uniprot Description
DLL4: Plays a role in the Notch signaling pathway. Activates Notch-1 and Notch-4. Binds to Notch-1 and Notch-4. Expressed in vascular endothelium.
Protein type: Cell development/differentiation; Membrane protein, integral
Cellular Component: integral to plasma membrane
Molecular Function: Notch binding; protein binding
Biological Process: angiogenesis; blood vessel remodeling; negative regulation of blood vessel endothelial cell migration; negative regulation of cell proliferation; negative regulation of Notch signaling pathway; negative regulation of transcription from RNA polymerase II promoter; Notch signaling pathway; patterning of blood vessels; positive regulation of Notch signaling pathway; regulation of neurogenesis; T cell differentiation
Research Articles on DLL4
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Product Notes
The Mouse DLL4 dll4 (Catalog #AAA9717853) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9717853 ELISA Kit recognizes Mouse DLL4. It is sometimes possible for the material contained within the vial of "Delta-Like Protein 4 (DLL4), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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