Typical Testing Data/Standard Curve (for reference only)
(Rat TNFSF12/TWEAK PicoKine ELISA Kit standard curve )
Rat TNFSF12/TWEAK ELISA Kit | TNFSF12 elisa kit
Rat TNFSF12/TWEAK PicoKine ELISA Kit
Gene Names
Tnfsf12; TWEAK
Reactivity
Rat
Synonyms
TNFSF12/TWEAK; Rat TNFSF12/TWEAK PicoKine ELISA Kit; Tumor necrosis factor ligand superfamily member 12; tumor necrosis factor (ligand) superfamily; member 12; APO 3 ligand; APO 3L; APO3 ligand; APO3/DR3 ligand; APO3L; CD255; DR3LG; MGC129581; MGC20669; TNF related weak inducer of apoptosis; TNF-related weak inducer of apoptosis; TNF12_HUMAN; TNFRS12a; TNFSF 12; TNFSF12; TNFSF12 protein; Tumor necrosis factor (ligand) superfamily member 12; Tumor necrosis factor superfamily member 12; TWEAK; UNQ181/PRO207; TNFSF12 elisa kit
Reactivity
Rat
Specificity
Natural and recombinant rat TNFSF12
Sequence Length
249
Samples
Cell culture supernates, serum and plasma (heparin, EDTA)
Assay Type
Sandwich
Detection Range
15.6pg/ml-1000pg/ml
Sensitivity
< 10pg/ml
Intra-assay Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-assay Precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Preparation and Storage
Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Typical Testing Data/Standard Curve (for reference only)
(Rat TNFSF12/TWEAK PicoKine ELISA Kit standard curve )
Typical Testing Data/Standard Curve (for reference only)
(Rat TNFSF12/TWEAK PicoKine ELISA Kit standard curve )
Related Product Information for TNFSF12 elisa kit
Principle of the assay: rat TNFSF12 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for TNFSF12 has been precoated onto 96-well plates. Standards(E.coli, S94-H249) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for TNFSF12 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the rat TNFSF12 amount of sample captured in plate.
Background: Tumor necrosis factor ligand superfamily member 12 also known as TNF-related weak inducer of apoptosis (TWEAK) is a protein that in humans is encoded by the TNFSF12 gene. The protein encoded by this gene is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This protein is a ligand for the FN14/TWEAKR receptor. This cytokine has overlapping signaling functions with TNF, but displays a much wider tissue distribution. This cytokine can induce apoptosis via multiple pathways of cell death in a cell type-specific manner. This cytokine is also found to promote proliferation and migration of endothelial cells, and thus acts as a regulator ofangiogenesis. The TNFSF12 gene lies 878 bp upstream of the putative transcriptional start site of the TNFSF13 gene on chromosome 17p13.1.
Background: Tumor necrosis factor ligand superfamily member 12 also known as TNF-related weak inducer of apoptosis (TWEAK) is a protein that in humans is encoded by the TNFSF12 gene. The protein encoded by this gene is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This protein is a ligand for the FN14/TWEAKR receptor. This cytokine has overlapping signaling functions with TNF, but displays a much wider tissue distribution. This cytokine can induce apoptosis via multiple pathways of cell death in a cell type-specific manner. This cytokine is also found to promote proliferation and migration of endothelial cells, and thus acts as a regulator ofangiogenesis. The TNFSF12 gene lies 878 bp upstream of the putative transcriptional start site of the TNFSF13 gene on chromosome 17p13.1.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
27,381 Da
NCBI Official Full Name
Tumor necrosis factor ligand superfamily member 12
NCBI Official Synonym Full Names
tumor necrosis factor ligand superfamily member 12
NCBI Official Symbol
Tnfsf12
NCBI Official Synonym Symbols
TWEAK
NCBI Protein Information
tumor necrosis factor ligand superfamily member 12; TNF-related weak inducer of apoptosis
UniProt Protein Name
Protein Tnfsf12
Protein Family
UniProt Gene Name
Prmt2
UniProt Entry Name
Q6AYC1_RAT
Research Articles on TNFSF12
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Product Notes
The Rat TNFSF12 prmt2 (Catalog #AAA177261) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA177261 ELISA Kit recognizes Rat TNFSF12. It is sometimes possible for the material contained within the vial of "TNFSF12/TWEAK, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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