Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Rabbit anti-Rat Delta Like Protein 4 (dLL4) Polyclonal Antibody | anti-dLL4 antibody
Polyclonal Antibody to Delta Like Protein 4 (dLL4)
Reactivity
Rat
Applications
Immunocytochemistry, Immunohistochemistry, ELISA, Western Blot
Purity
Affinity Chromatography
Synonyms
Delta Like Protein 4 (dLL4); Polyclonal Antibody; Polyclonal Antibody to Delta Like Protein 4 (dLL4); anti-dLL4 antibody
Host
Rabbit
Reactivity
Rat
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against dLL4. It has been selected for its ability to recognize dLL4 in immunohistochemical staining andwestern blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
Concentration
500ug/mL (varies by lot)
Sequence
Antigen: The target protein is fused with N-terminal His-Tag and its sequence is listed below.
MGHHHHHHSGSEF-GKYCDQP ICLSGCHEQN GYCSKPDECN CRPGWQGPLC NECIPHNGCR HGTCTIPWQC ACDEGWGGLF CDQDLNYCTH HSPCKNGSTC SNSGPRGYTC TCLPGYTGEH CELELSKCAS
MGHHHHHHSGSEF-GKYCDQP ICLSGCHEQN GYCSKPDECN CRPGWQGPLC NECIPHNGCR HGTCTIPWQC ACDEGWGGLF CDQDLNYCTH HSPCKNGSTC SNSGPRGYTC TCLPGYTGEH CELELSKCAS
Sequence Length
685
Applicable Applications for anti-dLL4 antibody
Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)
Application Notes
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Immunogen
Recombinant dLL4 (Gly214~Ser330) expressed in E.coli.
Cross Reactivity
Rat
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2081012
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Immunohistochemistry (IHC)
(DAB staining on fromalin fixed paraffin-embedded kidney tissue))
Immunohistochemistry (IHC)
(DAB staining on fromalin fixed paraffin-embedded kidney tissue))
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
74,961 Da
NCBI Official Full Name
delta-like protein 4 isoform X1
NCBI Official Synonym Full Names
delta like canonical Notch ligand 4
NCBI Official Symbol
Dll4
NCBI Protein Information
delta-like protein 4
UniProt Protein Name
Delta-like protein 4
Protein Family
UniProt Gene Name
Dll4
UniProt Synonym Gene Names
Delta4Curated
Uniprot Description
Involved in the Notch signaling pathway as Notch ligand. Activates NOTCH1 and NOTCH4 (PubMed:25700513). Involved in angiogenesis; negatively regulates endothelial cell proliferation and migration and angiogenic sprouting. Essential for retinal progenitor proliferation. Required for suppressing rod fates in late retinal progenitors as well as for proper generation of other retinal cell types. During spinal cord neurogenesis, inhibits V2a interneuron fate ().
Research Articles on dLL4
Similar Products
Product Notes
The dLL4 dll4 (Catalog #AAA2005913) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Delta Like Protein 4 (dLL4) reacts with Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Delta Like Protein 4 (dLL4) can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB). Western blotting: 1:100-400 Immunocytochemistry in formalin fixed cells: 1:100-500 Immunohistochemistry in formalin fixed frozen section: 1:100-500 Immunohistochemistry in paraffin section: 1:50-200 Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the dLL4 dll4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with N-terminal His-Tag and its sequence is listed below. MGHHHHHHSG SEF-GKYCDQ P ICLSGCHEQN GYCSKPDECN CRPGWQGPLC NECIPHNGCR HGTCTIPWQC ACDEGWGGLF CDQDLNYCTH HSPCKNGSTC SNSGPRGYTC TCLPGYTGEH CELELSKCAS. It is sometimes possible for the material contained within the vial of "Delta Like Protein 4 (dLL4), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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